A novel approach for antibody nanocarriers development through hydrophobic ion-pairing complexation
| Autor: | Ripal Gaudana, Ashaben Patel, Ashim K. Mitra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Taurocholic Acid
Sodium Pharmaceutical Science Nanoparticle chemistry.chemical_element Bioengineering Article chemistry.chemical_compound Immunoglobulin Fab Fragments Colloid and Surface Chemistry Organic chemistry Physical and Theoretical Chemistry Sodium dodecyl sulfate Drug Carriers Aqueous solution Organic Chemistry Dextran Sulfate Sodium Dodecyl Sulfate Taurocholic acid Nanostructures chemistry Immunoglobulin G Nanocarriers Drug carrier Hydrophobic and Hydrophilic Interactions Nuclear chemistry |
| Popis: | IgG-Fab fragment, a model antibody protein was hydrophobically modified by a novel approach of ion-pairing complexation. Three different sulphated ion-pairing agents were utilised including sodium dodecyl sulphate, taurocholic acid and dextran sulphate (DS). The formations of hydrophobic ion-pairing (HIP) complexes were dependant on pH and molar ratio of ion-pairing agent to Fab. Aqueous solubilities of HIP complexes were very low compared to Fab alone. In particular, when dextran sulphate was added as ion-pairing agent, formed Fab:DS HIP complexes were least soluble in water. Further, nanoparticles (NPs) loaded with drug and Fab:DS HIP complex were prepared and characterised with respect to encapsulation efficiency and size. We observed significant improvement in encapsulation efficiency for Fab:DS HIP complex-loaded nanoparticles. This study demonstrates a novel approach of formulating antibody-loaded nanoparticles which can also be employed for delivery of large antibodies. |
| Databáze: | OpenAIRE |
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