Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the members of physical activity discordant twin pairs
| Autor: | Eija Pöllänen, Tuulikki Seppänen-Laakso, Jaakko Kaprio, Heikki Kainulainen, Rita Rinnankoski-Tuikka, Urho M. Kujala, Vuokko Kovanen, Matej Orešič, P. Rahkila, Markku Alen, Mirva Rintala, Tuija Leskinen, Sarianna Sipilä |
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| Přispěvatelé: | Department of Public Health, Genetic Epidemiology |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
SELECTION FITNESS Twins Adipose tissue physical activity liikunta Physiology/Muscle and Connective Tissue Diabetes and Endocrinology/Obesity Cohort Studies chemistry.chemical_compound 0302 clinical medicine Risk Factors Adipocyte 311 Basic medicine geeniekspressio Finland Regulation of gene expression 0303 health sciences INSULIN-RESISTANCE Multidisciplinary Genetics and Genomics/Gene Expression Middle Aged geenien ilmentyminen 3. Good health medicine.anatomical_structure TRIACYLGLYCEROL Adipose Tissue Medicine SKELETAL-MUSCLE Female Public Health and Epidemiology/Exercise and Sports Research Article Muscle tissue medicine.medical_specialty Heart Diseases Cardiovascular Disorders Science 030209 endocrinology & metabolism EXERCISE Motor Activity Biology CAPACITY 03 medical and health sciences Insulin resistance SDG 3 - Good Health and Well-being Internal medicine metabolia medicine Humans Aerobic exercise Muscle Skeletal Cell Biology/Gene Expression Aged 030304 developmental biology matebolism MORTALITY Cholesterol HDL Skeletal muscle Lipid metabolism Lipid Metabolism medicine.disease kaksoset Endocrinology Gene Expression Regulation chemistry ADAPTATIONS CHRONIC DISEASE Energy Metabolism |
| Zdroj: | PLoS ONE, Vol 5, Iss 9 (2010) Leskinen, T, Rinnankoski-Tuikka, R, Rintala, M, Seppänen-Laakso, T, Pöllänen, E, Alen, M, Sipilä, S, Kaprio, J, Kovanen, V, Rahkila, P, Orešič, M, Kainulainen, H & Kujala, U M 2010, ' Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the members of physical activity discordant twin pairs ', PLoS ONE, vol. 5, no. 9, e12609 . https://doi.org/10.1371/journal.pone.0012609 PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | High physical activity/aerobic fitness predicts low morbidity and mortality. Our aim was to identify the most up-regulated gene sets related to long-term physical activity vs. inactivity in skeletal muscle and adipose tissues and to obtain further information about their link with cardio-metabolic risk factors. We studied ten same-sex twin pairs (age range 50-74 years) who had been discordant for leisure-time physical activity for 30 years. The examinations included biopsies from m. vastus lateralis and abdominal subcutaneous adipose tissue. RNA was analyzed with the genome-wide Illumina Human WG-6 v3.0 Expression BeadChip. For pathway analysis we used Gene Set Enrichment Analysis utilizing active vs. inactive co-twin gene expression ratios. Our findings showed that among the physically active members of twin pairs, as compared to their inactive co-twins, gene expression in the muscle tissue samples was chronically up-regulated for the central pathways related to energy metabolism, including oxidative phosphorylation, lipid metabolism and supportive metabolic pathways. Up-regulation of these pathways was associated in particular with aerobic fitness and high HDL cholesterol levels. In fat tissue we found physical activity-associated increases in the expression of polyunsaturated fatty acid metabolism and branched-chain amino acid degradation gene sets both of which associated with decreased 'high-risk' ectopic body fat and plasma glucose levels. Consistent with other findings, plasma lipidomics analysis showed up-regulation of the triacylglycerols containing the polyunsaturated fatty acids. Our findings identified skeletal muscle and fat tissue pathways which are associated with the long-term physical activity and reduced cardio-metabolic disease risk, including increased aerobic fitness. In particular, improved skeletal muscle oxidative energy and lipid metabolism as well as changes in adipocyte function and redistribution of body fat are associated with reduced cardio-metabolic risk. |
| Databáze: | OpenAIRE |
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