Poroelastic osmoregulation of living cell volume
| Autor: | Ali Akbar Alemrajabi, Andrea Malandrino, Emad Moeendarbary, Graham K. Sheridan, Guillaume Charras, Mohammad Hadi Esteki |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya. BBT - Grup de recerca en Biomaterials, Biomecànica i Enginyeria de Teixits |
| Rok vydání: | 2021 |
| Předmět: |
Cell biology
Multidisciplinary Osmotic concentration Chemistry Science Poromechanics Dynamics (mechanics) Biomecànica Article Rheology Volume (thermodynamics) Cytoplasm Cellular physiology Osmoregulation Biophysics Enginyeria biomèdica::Biomecànica [Àrees temàtiques de la UPC] Biomechanics Intracellular |
| Zdroj: | iScience iScience, Vol 24, Iss 12, Pp 103482-(2021) |
| ISSN: | 2589-0042 |
| Popis: | Summary Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeates through the membrane and cytoplasm. We investigated whether and how poroelasticity can describe volume dynamics in response to osmotic shocks. We exposed cells to osmotic perturbations and used defocusing epifluorescence microscopy on membrane-attached fluorescent nanospheres to track volume dynamics with high spatiotemporal resolution. We found that a poroelastic model that considers both geometrical and pressurization rates captures fluid-cytoskeleton interactions, which are rate-limiting factors in controlling volume changes at short timescales. Linking cellular responses to osmotic shocks and cell mechanics through poroelasticity can predict the cell state in health, disease, or in response to novel therapeutics. Graphical abstract Highlights • Cell height changes can be finely captured by defocusing microscopy • Water permeation and cellular deformability regulate dynamics of cell volume changes • Poroelasticity describes the dynamics of cell volume changes • The response of cell to hypo or hyperosmotic shocks are modeled by poroelasticity Cellular physiology; Cell biology; Biomechanics |
| Databáze: | OpenAIRE |
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