Deoxyribonuclease activity negative correlates with extracellular DNA in uncomplicated singleton pregnancies in the third trimester
| Autor: | Peter Celec, Jozef Záhumenský, Livia Melnikova, Ľubica Janovičová, Barbora Balažovjechová, Petra Psenkova, Barbora Vlková |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Pregnancy Trimester Third Preeclampsia Body Mass Index Andrology 03 medical and health sciences 0302 clinical medicine Pre-Eclampsia Pregnancy medicine Humans Fragmentation (cell biology) Correlation of Data 030304 developmental biology 0303 health sciences Fetus Deoxyribonucleases business.industry Obstetrics and Gynecology Reproducibility of Results Deoxyribonuclease activity medicine.disease Real-time polymerase chain reaction Cell-free fetal DNA Pediatrics Perinatology and Child Health Gestation Premature Birth Female business Cell-Free Nucleic Acids 030215 immunology Maternal Age |
| Zdroj: | Journal of perinatal medicineReferences. 49(6) |
| ISSN: | 1619-3997 |
| Popis: | Objectives It is not clear, which factors affect extracellular DNA (ecDNA) concentrations in healthy women with singleton uncomplicated pregnancies, although deoxyribonucleases (DNases) are hypothesized to be responsible for the cleavage of plasma ecDNA. The aim of this study was to analyze potential determinants of total ecDNA including plasma DNase activity. Methods Plasma samples were collected from 48 healthy women with singleton uncomplicated pregnancies in the third trimester (gestation week 37). DNA was isolated and quantified using fluorometry and real time PCR. DNase activity was assessed using the single radial enzyme-diffusion method. Results Neither ecDNA, nor DNase activity were affected by maternal age or BMI. DNase activity negatively correlated with total plasma ecDNA (r=−0.40, p=0.007). Similar associations were found for ecDNA of nuclear and mitochondrial origin, but not with fetal DNA quantified using Y-targeted PCR in male fetus-bearing pregnancies. Conclusions The role of plasma ecDNA of fetal and maternal origin is studied in the pathogenesis of pregnancy-complications. The results indicate that plasma DNase activity could negatively regulate ecDNA concentrations and should, thus, be analyzed in preeclampsia, preterm birth and other ecDNA-related pregnancy complications. |
| Databáze: | OpenAIRE |
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