Effects of oral administration of 5 immunosuppressive agents on activated T‐cell cytokine expression in healthy dogs

Autor: John M. Thomason, Andrew J. Mackin, Todd M. Archer, Charlee Mulligan, Crisanta Cruz-Espindola, Caitlin Riggs, Lakshmi Narayanan, Dawn M. Boothe, Roy Harmon, Claire L. Fellman, Robert W. Wills
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Drug
Interleukin 2
040301 veterinary sciences
T-Lymphocytes
media_common.quotation_subject
Immunology
Administration
Oral
Azathioprine
Standard Article
030204 cardiovascular system & hematology
Pharmacology
Mycophenolate
interleukin 2
0403 veterinary science
Interferon-gamma
Random Allocation
03 medical and health sciences
Dogs
0302 clinical medicine
Oral administration
Prednisone
Blood drug
medicine
Animals
cyclosporine
Leflunomide
media_common
leflunomide
Cross-Over Studies
azathioprine
lcsh:Veterinary medicine
General Veterinary
business.industry
mycophenolate mofetil
04 agricultural and veterinary sciences
Mycophenolic Acid
Standard Articles
interferon gamma
Interleukin-2
lcsh:SF600-1100
Female
SMALL ANIMAL
prednisone [Correction added on March 16
2020
after first online publication: See Appendix S1 for correction details.]
business
Immunosuppressive Agents
medicine.drug
Zdroj: Journal of Veterinary Internal Medicine, Vol 34, Iss 3, Pp 1206-1213 (2020)
Journal of Veterinary Internal Medicine
ISSN: 0891-6640
1939-1676
Popis: Background Dogs are often adminstered >1 immunosuppressive medication when treating immune‐mediated diseases, and determining whether these different medications affect IL‐2 expression would be useful when performing pharmacodynamic monitoring during cyclosporine therapy. Hypothesis/Objectives To determine the effects of 5 medications (prednisone, cyclosporine, azathioprine, mycophenolate mofetil, and leflunomide) on activated T‐cell expression of the cytokines IL‐2 and interferon‐gamma (IFN‐γ). Animals Eight healthy dogs. Methods Randomized, cross‐over study comparing values before and after treatment, and comparing values after treatment among drugs. Dogs were administered each drug at standard oral doses for 1 week, with a washout of at least 21 days. Activated T‐cell expression of IL‐2 and IFN‐γ mRNA was measured by quantitative reverse transcription polymerase chain reaction. Blood drug concentrations were measured for cyclosporine, mycophenolate, and leflunomide metabolites. Results Least squares means (with 95% confidence interval) before treatment for IL‐2 (2.91 [2.32‐3.50] ΔCt) and IFN‐γ (2.33 [1.66‐3.00 ΔCt]) values were significantly lower (both P
Databáze: OpenAIRE
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