A comparison of the sensitivity and specificity of enzyme immunoassays and time-resolved fluoroimmunoassay
| Autor: | Isabel M. Roberts, Stephen L. Jones, John C. Cox, Robert R. Premier |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Immunology
Fluoroimmunoassay Immunofluorescence Horseradish peroxidase Sensitivity and Specificity Immunoenzyme Techniques chemistry.chemical_compound Organophosphorus Compounds medicine Immunology and Allergy Humans Benzothiazoles Horseradish Peroxidase Hymecromone Acquired Immunodeficiency Syndrome ABTS Aniline Compounds medicine.diagnostic_test biology Phenolphthaleins Phenylpropionates Benzidines HIV Phosphate Alkaline Phosphatase Molecular biology Phenolphthalein chemistry Chromogenic Compounds biology.protein Alkaline phosphatase Enzyme immunoassays Sulfonic Acids |
| Zdroj: | Journal of immunological methods. 143(1) |
| ISSN: | 0022-1759 |
| Popis: | Time-resolved fluoroimmunoassay (TR-FIA) and various enzyme immunoassays (EIA) were compared in order to determine the detection system which showed the greatest degree of sensitivity without sacrificing specificity. The system chosen for the evaluation of these assays was the detection of antibodies to human immunodeficiency virus (HIV). For EIA, horseradish peroxidase (HRP) and alkaline phosphatase (AP) were investigated, each with a number of different substrates. HRP with its fluorogenic substrate, 3-(p-hydroxyphenyl)propionic acid (HPPA) was 1.6 times (p less than 0.01) more sensitive than with 3,3',5,5'-tetramethylbenzidine (TMB) and four times (p less than 0.001) more sensitive than with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS). AP with its fluorogenic substrate, 4-methylumbelliferyl phosphate (4MeUP), was 6-7 times (p less than 0.001) more sensitive than with phenolphthalein monophosphate (PMP) and 8-13 times (p less than 0.001) more sensitive than with p-nitrophenyl phosphate (pNPP). TR-FIA with Eu3(+)-labelled anti-human IgG was equivalent in sensitivity to HRP with TMB and AP with 4MeUP. |
| Databáze: | OpenAIRE |
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