Human chondrocyte proliferation and matrix synthesis cultured in Atelocollagen� gel
| Autor: | Kenichi Katsube, Masahiko Matsusaki, Mitsuo Ochi, Hideyuki Kurioka, Yuji Uchio |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Extracellular Matrix Proteins
Materials science Cell growth Cartilage Biomedical Engineering Biocompatible Materials In vitro Chondrocyte Cell biology Biomaterials Extracellular matrix Glycosaminoglycan Transplantation chemistry.chemical_compound Chondrocytes medicine.anatomical_structure chemistry medicine Humans Chondroitin Collagen Cell Division Cells Cultured Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research. 50:138-143 |
| ISSN: | 1097-4636 0021-9304 |
| Popis: | To evaluate the potential of Atelocollagen gel as a carrier for chondrocyte transplantation, histological and biochemical characteristics of the chondrocytes in gel culture were compared with those in conventional monolayer cultures. Articular chondrocytes from 20 patients were isolated by enzyme digestion, embedded in Atelocollagen gel, and cultured for up to 4 weeks. The effects on proliferation, morphological changes, and synthesis of proteoglycans were analyzed by cell counts, light and electron microscopy, and measurement of isomers of chondroitin sulfates. Chondrocytes embedded in the Atelocollagen gel gradually proliferated and produced chondroitin 6-sulfate, maintaining the chondrocyte phenotype for up to 4 weeks. In contrast, although monolayer chondrocytes increased in number, most could be characterized as being fibroblast-like cells with a reduced capability of producing chondroitin 6-sulfate. The results suggest that Atelocollagen gel permitted a gradual proliferation and matrix synthesis of chondrocytes and maintaining its phenotype. Atelocollagen gel represents an important carrier for the clinical application of cultured chondrocyte transplantation for repair of cartilage defects. |
| Databáze: | OpenAIRE |
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