Adaptive downregulation of a quinidine-sensitive cation conductance in renal principal cells of TWIK-1 knockout mice

Autor: H. C. Taylor, G. J. Cooper, Jacques Barhanin, L. Robson, Ian D. Millar, Jonathan D. Kibble
Přispěvatelé: Department of Biomedical Science, University of Sheffield [Sheffield], Medical Physiology Department, St. George's University [Grenada], Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2006
Předmět:
Male
Patch-Clamp Techniques
Physiology
Clinical Biochemistry
MESH: Mice
Knockout
MESH: Down-Regulation
Mice
MESH: Quinidine
0302 clinical medicine
MESH: Reverse Transcriptase Polymerase Chain Reaction
MESH: Animals
Epithelial polarity
Mice
Knockout
Membrane potential
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
[SDV.BA]Life Sciences [q-bio]/Animal biology
MESH: Potassium Channels
Tandem Pore Domain
Hyperpolarization (biology)
Adaptation
Physiological
Quinidine
Potassium channel
Barium
Knockout mouse
Female
medicine.medical_specialty
Down-Regulation
In Vitro Techniques
Biology
03 medical and health sciences
Potassium Channels
Tandem Pore Domain
Cations
Physiology (medical)
Internal medicine
MESH: Patch-Clamp Techniques
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Intercalated Cell
RNA
Messenger
Patch clamp
Kidney Tubules
Collecting
MESH: Cations
MESH: Kidney Tubules
Collecting
MESH: Mice
MESH: RNA
Messenger
030304 developmental biology
[SDV.GEN]Life Sciences [q-bio]/Genetics
MESH: Barium
Conductance
MESH: Adaptation
Physiological
MESH: Male
Endocrinology
MESH: Potassium
Potassium
Biophysics
MESH: Female
030217 neurology & neurosurgery
Zdroj: Pflügers Archiv European Journal of Physiology
Pflügers Archiv European Journal of Physiology, Springer Verlag, 2006, 453 (1), pp.107-16. ⟨10.1007/s00424-006-0107-0⟩
ISSN: 1432-2013
0031-6768
Popis: TWIK-1, a member of the two-pore domain K(+) channel family, is expressed in brain, kidney, and lung. The aim of this study was to examine the effect of loss of TWIK-1 on the renal cortical collecting duct. Ducts were isolated from wild-type and TWIK-1 knockout mice by enzyme digestion and whole-cell clamp obtained via the basolateral membrane. Current- and voltage-clamp approaches were used to examine K(+) conductances. No difference was observed between intercalated cells from wild-type or knockout ducts. In contrast, knockout principal cells were hyperpolarized compared to wild-type cells and had a reduced membrane conductance. This was a consequence of a fall in a barium-insensitive, quinidine-sensitive conductance (G (Quin)). G (Quin) demonstrated outward rectification and had a relatively low K(+) to Na(+) selectivity ratio. Loss of G (Quin) would be expected to lead to the hyperpolarization observed in knockout ducts by increasing fractional K(+) conductance and Na(+) uptake by the cell. Consistent with this hypothesis, knockout ducts had an increased diameter in comparison to wild-type ducts. These data suggest that G (Quin) contributes to the resting membrane potential in the cortical collecting duct and that a fall in G (Quin) could be an adaptive response in TWIK-1 knockout ducts.
Databáze: OpenAIRE
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