Data Processing Pipeline for Lipid Profiling of Carotid Atherosclerotic Plaque with Mass Spectrometry Imaging
| Autor: | Kim Van der Heiden, Antonius Fw van der Steen, Hence J.M. Verhagen, Heleen M.M. van Beusekom, Peter C. Burgers, Mirjam Visscher, Theo M. Luider, Gijs van Soest, Astrid M. Moerman |
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| Přispěvatelé: | Cardiology, Neurology, Surgery |
| Rok vydání: | 2019 |
| Předmět: |
Carotid Artery Diseases
Pathology medicine.medical_specialty medicine.medical_treatment Ischemia Method Endarterectomy Carotid endarterectomy 010402 general chemistry medicine.disease_cause 01 natural sciences Mass spectrometry imaging Imaging Structural Biology Image Processing Computer-Assisted medicine Humans MALDI-MSI Thrombus Stroke Vulnerable plaque Spectroscopy Artherosclerosis Plaque Electronic Data Processing Endarterectomy Carotid Mass spectrometry Chemistry 010401 analytical chemistry Fibrous cap Reproducibility of Results Thrombosis medicine.disease Lipids Plaque Atherosclerotic 0104 chemical sciences medicine.anatomical_structure Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Carotid artery Research Article |
| Zdroj: | Journal of the American Society for Mass Spectrometry, 30(9), 1790-1800. American Chemical Society Journal of the American Society for Mass Spectrometry |
| ISSN: | 1044-0305 |
| DOI: | 10.1007/s13361-019-02254-y |
| Popis: | Atherosclerosis is a lipid and inflammation-driven disease of the arteries that is characterized by gradual buildup of plaques in the vascular wall. A so-called vulnerable plaque, consisting of a lipid-rich necrotic core contained by a thin fibrous cap, may rupture and trigger thrombus formation, which can lead to ischemia in the heart (heart attack) or in the brain (stroke). In this study, we present a protocol to investigate the lipid composition of advanced human carotid plaques using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI), providing a framework that should enable the discrimination of vulnerable from stable plaques based on lipid composition. We optimized the tissue preparation and imaging methods by systematically analyzing data from three specimens: two human carotid endarterectomy samples (advanced plaque) and one autopsy sample (early stage plaque). We show a robust data reduction method and evaluate the variability of the endarterectomy samples. We found diacylglycerols to be more abundant in a thrombotic area compared to other plaque areas and could distinguish advanced plaque from early stage plaque based on cholesteryl ester composition. We plan to use this systematic approach to analyze a larger dataset of carotid atherosclerotic plaques. Electronic supplementary material The online version of this article (10.1007/s13361-019-02254-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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