Exploratory examination of inflammation state, immune response and blood cell composition in a human obese cohort to identify potential markers predicting cancer risk

Autor: Mariah Hay, Vivian Lam, Jordanna E. Kapeluto, William W. Mohn, Luke Bu, Brandon Cho, Gerald Krystal, Tom Elliott, Ingrid Elisia, William Jia, Hilary Leung, Michael Yu Li, David A. Harris
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Physiology
Type 2 diabetes
Pathology and Laboratory Medicine
Body Mass Index
Cohort Studies
White Blood Cells
0302 clinical medicine
Animal Cells
Neoplasms
Immune Physiology
Blood plasma
Medicine and Health Sciences
Medicine
Immune Response
Whole blood
Innate Immune System
Multidisciplinary
T Cells
Body Fluids
medicine.anatomical_structure
Blood
Physiological Parameters
030220 oncology & carcinogenesis
Cytokines
Female
Cellular Types
Anatomy
Research Article
Adult
T cell
Immune Cells
Science
Immunology
Risk Assessment
Blood Plasma
03 medical and health sciences
Immune system
Signs and Symptoms
Diagnostic Medicine
Humans
Obesity
Inflammation
Blood Cells
business.industry
Body Weight
Case-control study
Immunity
Cancer
Biology and Life Sciences
Red blood cell distribution width
Cell Biology
Molecular Development
medicine.disease
030104 developmental biology
Case-Control Studies
Immune System
business
Biomarkers
Developmental Biology
Granulocytes
Zdroj: PLoS ONE, Vol 15, Iss 2, p e0228633 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Obesity has reached epidemic proportions and is often accompanied by elevated levels of pro-inflammatory cytokines that promote many chronic diseases, including cancer. However, not all obese people develop these diseases and it would be very helpful to identify those at high risk early on so that preventative measures can be instituted. We performed an extensive evaluation of the effects of obesity on inflammatory markers, on innate and adaptive immune responses, and on blood cell composition to identify markers that might be useful in distinguishing those at elevated risk of cancer. Plasma samples from 42 volunteers with a BMI>35 had significantly higher CRP, PGE2, IL-1RA, IL-6 and IL-17 levels than 34 volunteers with normal BMIs. Of the cytokines and chemokines tested, only IL-17 was significantly higher in men with a BMI>35 than women with a BMI>35. As well, only IL-17 was significantly higher in those with a BMI>35 that had type 2 diabetes versus those without type 2 diabetes. Whole blood samples from participants with a BMI>35, when challenged with E. coli, produced significantly higher levels of IL-1RA while HSV-1 challenge resulted in significantly elevated IL-1RA and VEGF, and a non-significant increase in G-CSF and IL-8 levels. T cell activation of PBMCs, via anti-CD3 plus anti-CD28, resulted in significantly higher IFNγ production from volunteers with a BMI>35. In terms of blood cells, red blood cell distribution width (RDW), monocytes, granulocytes, CD4+T cells and Tregs were all significantly higher while, natural killer (NK) and CD8+ T cells were all significantly lower in the BMI>35 cohort, suggesting that obesity may reduce the ability to kill nascent tumor cells. Importantly, however, there was considerable person-to-person variation amongst participants with a BMI>35, with some volunteers showing markedly different values from controls and others showing normal levels of many parameters measured. These person-to-person variations may prove useful in identifying those at high risk of developing cancer.
Databáze: OpenAIRE
Externí odkaz: