G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep
| Autor: | Aubai O. Saleem, Amani Z Abdelgadir, Mohamed-Khair Aِbdalla Ahmed, Lutfi Mohamed-Ahmed Musa, Khaleel I. Jawasreh, Faisal M. El-Hag |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Litter (animal)
endocrine system Veterinary medicine Locus (genetics) Biology SF1-1100 03 medical and health sciences Polymorphism (computer science) Genotype SF600-1100 Sudanese sheep Allele Allele frequency similarity and identity 030304 developmental biology Genetics 0303 health sciences General Veterinary common ancestor growth differentiation factor 9 gene 0402 animal and dairy science Wild type 04 agricultural and veterinary sciences 040201 dairy & animal science Genotype frequency Animal culture Research Article |
| Zdroj: | Veterinary World Veterinary World, Vol 14, Iss 1, Pp 104-112 (2021) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression. |
| Databáze: | OpenAIRE |
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