Frequency of bacetrial content finding in persistant periapical lesions
| Autor: | Ana Ivanišević Malčić, Joško Grgurević, Arjana Tambić Andrašević, Goranka Prpić Mehičić, Silvana Jukić, Sania Kuzmac |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_treatment
Polymerase Chain Reaction law.invention Microbiology 03 medical and health sciences 0302 clinical medicine Prevotella nigrescens law 0502 economics and business medicine Treatment Failure General Dentistry Polymerase chain reaction Original Scientific Paper biology 05 social sciences 030206 dentistry Ribosomal RNA 16S ribosomal RNA biology.organism_classification korijenski kanal oblikovanje neuspjeh u liječenju periapeksni apsces bakterijska RNK 16S ribosomska RNK lančana reakcija polimerazom lcsh:RK1-715 Root Canal Preparation periapical abscess Bacterial RNA 16S Ribosomal RNA lcsh:Dentistry Apicoectomy Nucleic acid 050211 marketing Periapical Abscess Actinomyces |
| Zdroj: | Acta Stomatologica Croatica Acta Stomatologica Croatica, Vol 51, Iss 3, Pp 217-226 (2017) Acta stomatologica Croatica : International journal of oral sciences and dental medicine Volume 51 Issue 3 |
| ISSN: | 0001-7019 1846-0410 |
| Popis: | Ciljevi: Svrha ovoga rada bila je odrediti postotak perzistentnih apikalnih lezija pozitivnih na bakterijske nukleinske kiseline, zatim s pomoću lančane reakcije polimeraze detektirati mikroorganizme u periapikalnim lezijama koje je teško kultivirati te ih povezati s endodontskim neuspjehom, kliničkih simptomima i dijabetesom. Materijali i postupci: Uzorci perzistentnih apikalnih lezija skupljani su tijekom apikotomije. Bakterijska ubikvitarna početnica 16S rRNK rabljena je za otkrivanje 16S ribosomskog RNK u 36 uzoraka. PCR usmjeren na pojedine vrste proveden je s pomoću početnica za 16S rRNK gene Prevotelle nigrescens, Pseudoramibactera alactolyticusa i Propionobacterium propionicum. Rezultati: Šest uzoraka (16,67 %) bilo je pozitivno na bakterijski ribosomski RNK. Pseudoramibacter alactolyticus detektiran je u trima uzorcima. Propionibacterium propionicum i Prevotella nigrescens detektirani su svaki u po jednom uzorku. Prevalencija infekcije ovih lezija P. intermedia, P. propionicum i P. alactolyticusom niska je. Provedeno istraživanje nije dalo dostatne podatke o povezanosti ekstraradikularne infekcije s dijabetesom melitusom i kliničkim simptomima. Zaključci: Apikalne lezije koje perzistiraju nakon endodontskog liječenja mogu, uz Actinomyces i Propionicum species, sadržavati i druge mikroorganizme. Objectives: To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and diabetes mellitus. Materials and methods: The samples of persistent apical lesions were collected during apicoectomy. Bacterial ubiquitous primer 16S rRNA was used to detect 16S ribosomal RNA in 36 samples. A species–specific PCR was performed with primers targeted to the bacterial 16S rRNA genes of Prevotella Nigrescens, Pseudoramibacter alactolyticus, and Propionobacterium propionicum. Results: Six samples (16.67%) were positive for bacterial ribosomal RNA. Pseudoramibacter alactolyticus was detected in three samples. Propionibacterium propionicum and Prevotella nigrescens were detected in one sample each. The prevalence of infection of such lesions with P. intermedia, P. propionicum and P. alactolyticus is low. Conslusion: The study we conducted gave insufficient data about extraradicular infection and its connection with diabetes mellitus and clinical symptoms. Conclusions: Apical lesions persisting after endodontic treatment could harbor microorganisms other than Actinomyces and Propionicum species. |
| Databáze: | OpenAIRE |
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