Purification and partial characterization of a novel human platelet aggregation factor in the extracellular products ofStreptococcus mitis, strain Nm-65
| Autor: | Nobutaka Ohtani, Shozo Kotani, Toshio Ishikawa, Yuko Todome, Fuminori Okibayashi, Goro Asano, Yukino Watanabe, Hisashi Ohkuni |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Microbiology (medical) Platelet Aggregation Immunology Microbiology Bacterial Proteins Streptococcus mitis Extracellular Humans Immunology and Allergy Platelet Platelet Activating Factor Molecular mass Strain (chemistry) biology Streptococcus Biological activity General Medicine Middle Aged biology.organism_classification Streptococcaceae Molecular biology Infectious Diseases Biochemistry Female Extracellular Space Platelet Aggregation Inhibitors Bacteria |
| Zdroj: | FEMS Immunology & Medical Microbiology. 17:121-129 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | A human blood platelet aggregation factor was purified from the extracellular products (ECP) of Streptococcus mitis, strain Nm-65 by sequential chromatography on DEAE-Sepharose CL-6B, hydroxyapatite and Superdex 75 columns. The purified factor (S. mitis-derived human platelet aggregation factor, Sm-hPAF) gave a single band with a molecular weight of 66 kDa on SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Sm-hPAF showed a peak absorption at 278 nm and an isoelectric point of around 8.5. Chemical analyses revealed that Sm-hPAF contained no sugars and that its first 15 amino-terminal amino acid residues were H-DEQGNRPVETENIAR. Platelet aggregation activity of Sm-hPAF was abolished by heating at 45 degrees C for 10 min. Platelet aggregation by Sm-hPAF was accompanied by a release of prostaglandin E2 (PGE2) in a dose-dependent manner. The platelet aggregation was not inhibited by either prostaglandin E1 (PGE1) or Gly-Arg-Gly-Asp-Ser (GRGDS), that inhibit the platelet aggregation induced by collagen. Twenty (77%) platelet rich-plasma (PRP) specimens derived from 26 healthy volunteers were aggregated by Sm-hPAF, but the remaining 6 (23%) were not reactive. A preliminary study suggested the presence of an inhibitory factor against Sm-hPAF in the plasma from a non-reactive donor. |
| Databáze: | OpenAIRE |
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