Comparison of the effect of two different handling media on rabbit zygote developmental ability
| Autor: | M.J Escribá, Ayman M. Saeed, Miguel Á. Silvestre, F. García-Ximénez |
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| Předmět: |
Embryology
Zygote Medicine (miscellaneous) Biology Andrology Pregnancy In vivo Culture Techniques medicine Animals Blastocyst Incubation Embryogenesis Uterine horns Anatomy Embryo Transfer medicine.disease Oocyte Culture Media medicine.anatomical_structure Reproductive Medicine Female Animal Science and Zoology Rabbits Developmental Biology Food Science |
| Zdroj: | ResearcherID |
| Popis: | Despite the attention paid to culture media, the relevance of the handling medium at egg recovery/transfer is frequently overlooked. In the present work, we compare the effect of two different handling media (PBS and HEPES-buffered Ham F10, both supplemented with 20% (v/v) FCS), upon in vitro and in vivo developmental ability of in vivo fertilised rabbit zygotes. Zygotes recovered in HEPES-buffered medium (permanence 1 h as maximum) and subsequently cultured in vitro devel- oped more efficiently to the compacted morula (100%) and blastocyst stage (92%) than those recov- ered in PBS (83% and 76%, respectively, P < 0.05). Zygotes recovered in such media were then further bilaterally transferred to recipient does following a brief in vitro culture period (for 4 hours). At caesarean section (day 28 of pregnancy), significant differences were observed in both the percentage of pregnant uterine horns (PBS: 60% vs. HEPES-buffered Ham F10: 100%) and live birth rates (PBS: 14% vs. HEPES-buffered Ham F10: 34%). Thus when early rabbit zygotes must be handled, even for short incubation periods, the medium is not innocuous. culture media / buffer system / development / zygote / rabbit |
| Databáze: | OpenAIRE |
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