A Rosemary Extract Rich in Carnosic Acid Selectively Modulates Caecum Microbiota and Inhibits β-Glucosidase Activity, Altering Fiber and Short Chain Fatty Acids Fecal Excretion in Lean and Obese Female Rats

Autor: Rocío García-Villalba, Francisco A. Tomás-Barberán, María-Teresa García-Conesa, Mar Larrosa, María Romo-Vaquero, María Obiol, Marc Roller, John G. Flanagan, Rocío González-Barrio, María-Victoria Selma, Nicolas Issaly
Přispěvatelé: Centro para el Desarrollo Tecnológico Industrial (España), Ministerio de Economía y Competitividad (España), Fundación Séneca
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Dietary Fiber
Physiology
Enzyme Metabolism
Gut flora
Biochemistry
Cecum
chemistry.chemical_compound
Feces
Lactobacillus
Microbial Physiology
Medicine and Health Sciences
Enzyme Chemistry
Immune Response
Mammals
Multidisciplinary
biology
beta-Glucosidase
Fatty Acids
Clostridium leptum
Microbial Growth and Development
Animal Models
Organ Size
Lipids
Enzymes
medicine.anatomical_structure
Physiological Parameters
Vertebrates
Medicine
Female
Research Article
medicine.medical_specialty
Science
Immunology
Research and Analysis Methods
digestive system
Rodents
Microbiology
Caecum
Excretion
Model Organisms
Internal medicine
medicine
Animals
Obesity
Microbial Metabolism
Nutrition
Inflammation
Plant Extracts
Body Weight
Immunity
Organisms
Biology and Life Sciences
Carnosic acid
Metabolism
biology.organism_classification
Fatty Acids
Volatile
Hormones
Rosmarinus
Rats
Rats
Zucker
Endocrinology
chemistry
Metabolic Disorders
Abietanes
Enzymology
alpha-Amylases
Developmental Biology
Zdroj: PLoS ONE
Digital.CSIC. Repositorio Institucional del CSIC
instname
PLoS ONE, Vol 9, Iss 4, p e94687 (2014)
ISSN: 1932-6203
Popis: [Background] Carnosic acid (CA) and rosemary extracts (RE) show body-weight, energy metabolism and inflammation regulatory properties in animal models but the mechanisms are not yet understood. Gut microbiota plays an important role in the host metabolism and inflammatory status and is modulated by the diet. The aim of this research was to investigate whether a RE enriched in CA affected caecum microbiota composition and activity in a rat model of genetic obesity.
[Methods and Principal Findings] A RE (40% CA) was administered with the diet (0.5% w/w) to lean (fa/+) and obese (fa/fa) female Zucker rats for 64 days. Changes in the microbiota composition and β-glucosidase activity in the caecum and in the levels of macronutrients and short chain fatty acids (SCFA) in feces were examined. The RE increased the Blautia coccoides and Bacteroides/Prevotella groups and reduced the Lactobacillus/Leuconostoc/Pediococccus group in both types of animals. Clostridium leptum was significantly decreased and Bifidobacterium increased only in the lean rats. β-Glucosidase activity was significantly reduced and fecal fiber excretion increased in the two genotypes. The RE also increased the main SCFA excreted in the feces of the obese rats but decreased them in the lean rats reflecting important differences in the uptake and metabolism of these molecules between the two genotypes.
[Conclusions] Our results indicate that the consumption of a RE enriched in CA modifies microbiota composition and decreases β-glucosidase activity in the caecum of female Zucker rats while it increases fiber fecal elimination. These results may contribute to explain the body weight gain reducing effects of the RE. The mutated leptin receptor of the obese animals significantly affects the microbiota composition, the SCFA fecal excretion and the host response to the RE intake.
This project was financed by the Spanish Centre for the Development of Industrial Technology (CDTI) as part of the project SENIFOOD, which belongs to the CENIT subvention program (Ref: CEN-20091006). This work was also supported by the Project Consolider Ingenio 2010, CSD2007-00063 (Fun-C-Food) and by the Seneca Foundation of the Region of Murcia, Spain (Group of Excellence GERM 06 04486 and 05556/PI/04).
Databáze: OpenAIRE
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