Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns
| Autor: | Simon Tomlinson, Alan Sher, Caetano Reis e Sousa, Damien Chaussabel, Oliver Schulz, Alexander D. Edwards |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Microarray
Cellular differentiation Immunology Information Storage and Retrieval CD11c chemical and pharmacologic phenomena Cell Separation Biology Immunophenotyping Mice Databases Genetic Gene expression Animals Immunology and Allergy RNA Messenger CD72 Gene Oligonucleotide Array Sequence Analysis Genetics Microarray analysis techniques Gene Expression Profiling Computational Biology hemic and immune systems Dendritic Cells Dendritic cell Molecular biology CD11c Antigen Mice Inbred C57BL Multigene Family Spleen |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.171.1.47 |
| Popis: | The functional relationships and properties of different subtypes of dendritic cells (DC) remain largely undefined. To better characterize these cells, we used global gene analysis to determine gene expression patterns among murine CD11chigh DC subsets. CD4+, CD8α+, and CD8α− CD4− (double negative (DN)) DC were purified from spleens of normal C57/BL6 mice and analyzed using Affymetrix microarrays. The CD4+ and CD8α+ DC subsets showed distinct basal expression profiles differing by >200 individual genes. These included known DC subset markers as well as previously unrecognized, differentially expressed CD Ags such as CD1d, CD5, CD22, and CD72. Flow cytometric analysis confirmed differential expression in nine of nine cases, thereby validating the microarray analysis. Interestingly, the microarray expression profiles for DN cells strongly resembled those of CD4+ DC, differing from them by |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|