A Comparison of Two Buffering Systems for Lens Organ Culture
| Autor: | Klaus-Peter Pollmann, Inge Korte, Klaus-Peter Brenner |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
genetic structures
Phosphofructokinase-1 Bicarbonate Buffers Biology Organ culture law.invention Cellular and Molecular Neuroscience chemistry.chemical_compound Adenosine Triphosphate Organ Culture Techniques law Adenine nucleotide Lens Crystalline Animals Incubation HEPES General Medicine Adenosine Monophosphate eye diseases Sensory Systems Culture Media Lens (optics) Bicarbonates Ophthalmology chemistry Biochemistry Cattle Specific activity sense organs Phosphofructokinase |
| Zdroj: | Ophthalmic Research. 14:265-268 |
| ISSN: | 1423-0259 0030-3747 |
| Popis: | Bovine lenses were incubated in a pairwise assay for 24 h in TC 199 which was buffered with Hepes or NaHCO3, respectively. In lenses incubated with Hepes, the content of ATP was higher and that of AMP lower than in those incubated with NaHCO3. The specific activity of phosphofructokinase (PFK), was higher in the lenses from the Hepes medium than in lenses incubated with NaHCO3. A heat-labile metazyme of the PFK which is found in the nucleus of unincubated lenses disappeared when the lenses were incubated in the presence of bicarbonate, but remained intact after 22 h incubation with Hepes. |
| Databáze: | OpenAIRE |
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