CRISPR-dCas9 based DNA detection scheme for diagnostics in resource-limited settings

Autor:	Michel Bengtson, Mitasha Bharadwaj, Oskar Franch, Jaco van der Torre, Veronique Meerdink, Henk Schallig, Cees Dekker
Přispěvatelé:	Medical Microbiology and Infection Prevention
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Point-of-Care Testing Clustered Regularly Interspaced Short Palindromic Repeats General Materials Science DNA Nucleic Acid Amplification Techniques RNA Guide Kinetoplastida
Zdroj:	Nanoscale, 14(5) Nanoscale, 14(5), 1885-1895. Royal Society of Chemistry Nanoscale 14
ISSN:	2040-3372
Popis:	Nucleic-acid detection is crucial for basic research as well as for applications in medicine such as diagnostics. In resource-limited settings, however, most DNA-detection diagnostic schemes are inapplicable since they rely on expensive machinery, electricity, and trained personnel. Here, we present an isothermal DNA detection scheme for the diagnosis of pathogenic DNA in resource-limited settings. DNA was extracted from urine and blood samples using two different instrument-free methods, and amplified using Recombinase Polymerase Amplification with a sensitivity of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3fbc960c21c5a6f53bf1655e5018f38 http://www.scopus.com/inward/record.url?scp=85124056054&partnerID=8YFLogxK Zobrazit plný text záznamu