In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus
| Autor: | Azeez Sait Sahul Hameed, Shanmugam Santhosh Kumar, Chakalaparambil Gokulan Rakesh, Ravi Nanthini, Selvam Sivakumar, Devika Pillai, Seepoo Abdul Majeed, S. Vimal, Kalasseril Girijan Sneha, G. Taju |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Oreochromis mossambicus food.ingredient Veterinary (miscellaneous) Spleen Aquatic Science Biology medicine.disease_cause Cryopreservation law.invention Cell Line 03 medical and health sciences Fish Diseases food RNA Virus Infections law medicine Animals RNA Viruses Polymerase chain reaction Tilapia lake virus Tilapia 04 agricultural and veterinary sciences biology.organism_classification Molecular biology 030104 developmental biology medicine.anatomical_structure Cell culture 040102 fisheries 0401 agriculture forestry and fisheries Disease Susceptibility Epithelioid cell |
| Zdroj: | Journal of fish diseasesREFERENCES. 42(11) |
| ISSN: | 1365-2761 |
| Popis: | Tilapia lake virus (TiLV)-suspected samples of tilapia were collected from grow-out ponds located with clinical signs and mortality ranged from 5% to 50%. The reverse transcription-polymerase chain reaction (RT-PCR) assay revealed the presence of TiLV in the disease outbreak ponds. Cell lines were developed from heart, gill and eye of Mozambique tilapia and characterized. Morphologically, these cell lines are composed of epithelioid cells. The optimum growth of these cells was observed at 28°C and 20% concentration of FBS. After cryopreservation, 70%-90% of cells were found to be viable. The cells of all three cell lines were found to be positive to fibronectin and pancytokeratin. PCR amplification of 16S rRNA and COI of O. mossambicus confirmed the origin of these cell lines from O. mossambicus. Heart and gill cell lines were found to be highly susceptible to TiLV and found to be useful for its isolation from infected fish samples. The experimental infection was carried out in O. niloticus and O. mossambicus using the TiLV propagated in susceptible cell lines. The RT-PCR results revealed the presence of TiLV in brain, gill, liver, kidney, spleen, eye, muscle, intestine and heart of experimentally infected O. niloticus and O. mossambicus. |
| Databáze: | OpenAIRE |
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