High-pressure liquid chromatography of cobalamins and cobalamin analogs
| Autor: | Kenneth C. Van Horne, Robert H. Allen, J. Fred Kolhouse, Mitchell Binder |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Chromatography
Chemical Phenomena Chemistry Biophysics Cell Biology Sulfitocobalamin Hydroxocobalamin Biochemistry High-performance liquid chromatography Cobalamin Adenosylcobalamin Vitamin B 12 chemistry.chemical_compound Methylcobalamin polycyclic compounds medicine Cyanocobalamin Molecular Biology Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Analytical Biochemistry. 125:253-258 |
| ISSN: | 0003-2697 |
| Popis: | High-pressure liquid chromatography has been used to separate, identify, and quantitate 37 different cyanocobalamin analogs, including the most commonly occurring analogs that result from bacterial synthesis. This technique has also been used to simultaneously separate, identify, and quantitate five naturally occurring cobalamins that differ in their upper axial ligands: methylcobalamin, adenosylcobalamin, hydroxocobalamin, cyanocobalamin, and sulfitocobalamin. This method permits rapid quantitative detection and identification of cobalamins and naturally occurring and synthetic cobalamin analogs in complex mixtures. |
| Databáze: | OpenAIRE |
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