Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice
| Autor: | Artur Kamiński, Piotr Fiedor, Hady Razak Hady, Grażyna Przybytniak, Izabela Poplawska, Alicja Walewska, Marcin Moniuszko, Jarosław Sadło, Kamil Grubczak, Tomasz Oldak, Sławomir Lech Czaban, Wojciech Głuszewski, Jordan Holl, Zbigniew Zimek, Andrzej Eljaszewicz, Hanna Lewandowska, Marlena Tynecka, Małgorzata Dąbrowska-Gralak, Cezary Kowalewski, Joanna Reszeć |
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| Rok vydání: | 2021 |
| Předmět: |
collagen
Free Radicals QH301-705.5 ADM Catalysis Article law.invention Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine law Skin substitutes Radiation Sterilization Humans Acellular Dermis Irradiation Biology (General) Physical and Theoretical Chemistry Electron paramagnetic resonance QD1-999 Molecular Biology Spectroscopy 030304 developmental biology 0303 health sciences biological dressing Chemistry Organic Chemistry Sterilization radiation sterilization General Medicine Sterilization (microbiology) Bandages 3. Good health Computer Science Applications Clinical Practice Critical parameter Gamma Rays skin substitutes 030220 oncology & carcinogenesis human acellular dermal matrices Dermal dressing Biomedical engineering |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 16 International Journal of Molecular Sciences, Vol 22, Iss 8467, p 8467 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22168467 |
| Popis: | Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential. |
| Databáze: | OpenAIRE |
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