Effects of rhamnolipid on the cellulase and xylanase in hydrolysis of wheat straw

Autor: Chun-Hua Li, Hong-Yuan Wang, Shuang Liu, Min Li, Bing-Quan Fan
Rok vydání: 2010
Předmět:
Zdroj: Bioresource technology. 102(11)
ISSN: 1873-2976
Popis: The effects of biosurfactant rhamnolipid (RL) and chemical surfactant Triton X-100 on the production of cellulases and xylanase from Penicillium expansum (P. expansum) in untreated, acid- and alkali-pretreated wheat straw submerged fermentations were studied, and the influences on the activity and stability of Cellulase R-10 were also investigated. The results showed that RL and Triton X-100 enhanced the activities of cellulases and xylanase to different extents and the stimulatory effects of RL were superior to those of Triton X-100. During the peak enzyme production phase, RL (60 RE mg/l) increased cellulases activities by 25.5–102.9%, in which the raise of the same enzyme in acid-pretreated straw broths was the most. It was found that the reducing sugars by hydrolyzing wheat straw with Cellulase R-100 were not visibly increased after adding RL. However, it distinctly protected Cellulase R-10 from degradation or inactivation, keeping the reducing sugars yield at about 17%. 2011 Published by Elsevier Ltd.
Databáze: OpenAIRE