In Situ Visualization of hERG Potassium Channel via Dual Signal Amplification
| Autor: | Kai Zhang, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
congenital hereditary and neonatal diseases and abnormalities Surface Properties hERG Regulator Metal Nanoparticles 01 natural sciences Analytical Chemistry Cell membrane 03 medical and health sciences medicine Humans cardiovascular diseases Particle Size Cells Cultured biology 010405 organic chemistry Chemistry HEK 293 cells Nucleic acid amplification technique Potassium channel Ether-A-Go-Go Potassium Channels 0104 chemical sciences 030104 developmental biology medicine.anatomical_structure HEK293 Cells Rolling circle replication Spherical nucleic acid biology.protein Biophysics MCF-7 Cells Gold Nucleic Acid Amplification Techniques |
| Zdroj: | Analytical chemistry. 90(10) |
| ISSN: | 1520-6882 |
| Popis: | Dysfunction of the human ether-a-go-go related gene (hERG)-encoded potassium channel is identified as a major cause of the long QT syndrome, a marker for lethal cardiac arrhythmia. Furthermore, recent studies revealed that hERG K+ channel is a regulator of tumor cell apoptosis and proliferation. Herein, an ultrasensitive fluorescence assay combining DNA-functionalized gold nanoparticles and rolling circle amplification (RCA) was attempted for the first time to visualize hERG channels in living cells. The spherical nucleic acid gold nanoparticles, which can anchor on hERG channels in the cell membrane, not only act as the primary amplification elements but also trigger the subsequent RCA reaction to achieve the secondary amplification. Within 30 min, the ratio of reporter to target can reach up to 104, realizing the detection of hERG channels in cells with low-level expression. Therefore, the strategy provides a valuable tool for hERG-related studies. More importantly, it opens a new horizon for imaging va... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|