The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium

Autor:	Yanyan Zhu, Yanan Jiang, Yanyan Xu, Simin Zhao, Jing Lu, Lili Zhu, Xinhuan Chen, Jimin Zhao, Junfen Ma, Ge Jin, Jiace Qin, Tingting Niu, Kangdong Liu, Ziming Dong
Rok vydání:	2020
Předmět:	Pathology medicine.medical_specialty Article Subject General Immunology and Microbiology business.industry H&E stain General Medicine Precancerous lesion Esophageal cancer medicine.disease female genital diseases and pregnancy complications General Biochemistry Genetics and Molecular Biology Pathogenesis Esophageal epithelium Conditional gene knockout medicine Medicine Immunohistochemistry business Pathological
Zdroj:	BioMed Research International, Vol 2020 (2020)
ISSN:	2314-6141 2314-6133
DOI:	10.1155/2020/4534289
Popis:	Understanding the molecular mechanisms of precancerous lesion of esophageal cancer is beneficial for early diagnosis and early treatment. The deletion of p53 gene is common in esophageal cancer, but its pathogenesis is still unclear. An animal model is urgently needed to study the mechanisms of esophageal cancer and p53 deficiency. KO mice (p53flox/flox.ED-L2-Cre+/−) and the corresponding control Loxp mice (p53flox/flox.ED-L2-Cre−/−) were obtained by crossing between the p53flox/flox mice and ED-L2-Cre+/− mice. Methylbenzylnitrosamine (NMBA) was injected subcutaneously to induce esophageal precancerous lesion of these two groups of mice. Hematoxylin and eosin staining analysis was performed to evaluate the number and extent of esophageal precancerous lesions in KO mice and Loxp mice at the 16th and 48th weeks. Immunohistochemistry analysis was used to detect the change of Ki67, P21, Bcl-2, and Bax proteins. The number and extent of esophageal precancerous lesions in KO mice were significantly increased compared with the control at the 16th and 48th weeks under the induction of NMBA. The Ki67, P21, Bcl-2, and Bax proteins also had cancer-related pathological characteristics. These results suggest that the esophageal precancerous lesion model was established under the combined effect of p53 gene deletion in esophageal epithelium and NMBA, which could provide a new esophageal precancerous lesion model to explore the mechanism of precancerous lesions.
Databáze:	OpenAIRE
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