The Frequency of PON1 L55M Polymorphism and Measurement of Oxidative Stress Parameters (Superoxide Dismutase, Catalase, Thiol, and Carbonyl) in Neonates with G6PD Deficiency Compared to the Control Group
| Autor: | Vahid Pourshafiei, Ali Moradi, Vahide Jamshidi, Mahmoud Vakili |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty lcsh:R5-920 biology business.industry medicine.disease_cause PON1 g6pd pon1 polymorphism Superoxide dismutase Endocrinology chemistry Polymorphism (materials science) Catalase Internal medicine neonatal Thiol biology.protein Medicine oxidative stress business lcsh:Medicine (General) Oxidative stress |
| Zdroj: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd, Vol 27, Iss 12, Pp 2203-2214 (2019) |
| ISSN: | 2228-5733 2228-5741 |
| Popis: | Introduction: Glucose-6-phosphate dehydrogenase deficiency is the most common enzyme disorder. This enzyme involved in maintaining the balance of active oxygen species and its defect causes oxidative damage. PON1 is an HDL-based glycosylated protein that prevents lipid peroxidation. In this study, the prevalence of PON L55M polymorphism in paraoxonase enzyme in neonates with a deficiency in G6PD activity was evaluated, and the level of oxidative stress was measured. Methods: In the present case-control study, 60 infants 2 to 6 months with G6PD enzyme activity deficiency and 60 healthy infants identical in age was selected. Polymorphism examination was done using PCR-RFLP technique, and oxidative stress parameters were measured by spectrophotometry. Chi square and t test statistical analysis of data was performed using SPSS V16 software. Results: The frequency of genotype LL, LM and MM for PON1 L55M was 43.33%, 43.3% and 13.3% and, 35%, 21.6% and 43.3% in control and case group, respectively. Genotypic frequency of LM and MM was significant between control and control groups (P 0.05), but the activity of catalase enzyme and mean level of thiol was showed a significant difference (P |
| Databáze: | OpenAIRE |
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