Collagen type X is essential for successful mesenchymal stem cell-mediated cartilage formation and subsequent endochondral ossification
| Autor: | Yanto Ridwan, J van de Peppel, Niamh Fahy, Eric Farrell, Janneke Witte-Bouma, Roberto Narcisi, E. Andrés Sastre, M J Koudstaal, E M Strabbing, Callie Knuth, Eppo B. Wolvius |
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| Přispěvatelé: | Oral and Maxillofacial Surgery, Orthopedics and Sports Medicine, Molecular Genetics, Radiology & Nuclear Medicine, Internal Medicine |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:Diseases of the musculoskeletal system
0206 medical engineering lcsh:Surgery regenerative medicine Mice Nude 02 engineering and technology Cartilage metabolism Regenerative medicine Extracellular matrix Mice chondrogenesis Chondrocytes Tissue engineering In vivo Osteogenesis Animals Humans bone tissue engineering Child Endochondral ossification Cells Cultured Mice Inbred BALB C Chemistry Mesenchymal stem cell Mesenchymal Stem Cells lcsh:RD1-811 Chondrogenesis 020601 biomedical engineering Cell biology endochondral ossification Cartilage lcsh:RC925-935 Collagen Type X |
| Zdroj: | European Cells and Materials European cells & materials, 38, 106-122. AO Research Institute European Cells & Materials, Vol 38, Pp 106-122 (2019) |
| ISSN: | 1473-2262 |
| Popis: | in tissue engineering, endochondral ossification (EO) is often replicated by chondrogenically differentiating mesenchymal stromal cells (MSCs) in vitro and achieving bone formation through in vivo implantation. The resulting marrow-containing bone constructs are promising as a treatment for bone defects. However, limited bone formation capacity has prevented them from reaching their full potential. This is further complicated since it is not fully understood how this bone formation is achieved. Acellular grafts derived from chondrogenically differentiated MSCs can initiate bone formation; however, which component within these decellularised matrices contribute to bone formation has yet to be determined. Collagen type X (COLX), a hypertrophy-associated collagen found within these constructs, is involved in matrix organisation, calcium binding and matrix vesicle compartmentalisation. However, the importance of COLX during tissue-engineered chondrogenesis and subsequent bone formation is unknown. The present study investigated the importance of COLX by shRNA-mediated gene silencing in primary MSCs. A significant knock-down of COLX disrupted the production of extracellular matrix key components and the secretion profile of chondrogenically differentiated MSCs. Following in vivo implantation, disrupted bone formation in knock-down constructs was observed. The importance of COLX was confirmed during both chondrogenic differentiation and subsequent EO in this tissue engineered setting. |
| Databáze: | OpenAIRE |
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