Dynamic microtubules are essential for efficient chromosome capture and biorientation in S. cerevisiae
| Autor: | Baoying Huang, Tim C. Huffaker |
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| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular Saccharomyces cerevisiae Proteins Kinetochore Biorientation Aurora B kinase Mitosis Saccharomyces cerevisiae Spindle Apparatus Cell Biology Biology Microtubules Models Biological Spindle pole body Cell biology Spindle apparatus Spindle checkpoint Tubulin Report Mutation Chromosomes Fungal Kinetochores Astral microtubules Research Articles |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Attachment of chromosomes to the mitotic spindle has been proposed to require dynamic microtubules that randomly search three-dimensional space and become stabilized upon capture by kinetochores. In this study, we test this model by examining chromosome capture in Saccharomyces cerevisiae mutants with attenuated microtubule dynamics. Although viable, these cells are slow to progress through mitosis. Preanaphase cells contain a high proportion of chromosomes that are attached to only one spindle pole and missegregate in the absence of the spindle assembly checkpoint. Measurement of the rates of chromosome capture and biorientation demonstrate that both are severely decreased in the mutants. These results provide direct evidence that dynamic microtubules are critical for efficient chromosome capture and biorientation and support the hypothesis that microtubule search and capture plays a central role in assembly of the mitotic spindle. |
| Databáze: | OpenAIRE |
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