Agrobacterium rhizogenes rolA gene promotes tolerance to Fusarium oxysporum f. sp. lycopersici in transgenic tomato plants (Solanum lycopersicum L.)
| Autor: | Rita Baraldi, Francesca Rapparini, Enrico Santangelo, P. Bettini, Pietro Mosconi, Maria Luisa Mauro, Paola Crinò |
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| Přispěvatelé: | Crinò, P. |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agrobacterium Transgene Plant Science Genetically modified crops tomato defence response Fusarium oxysporum rolA gene 01 natural sciences 03 medical and health sciences chemistry.chemical_compound ABA Defence response IAA Tomato Botany Genetically modified tomato Abscisic acid biology fungi food and beverages biology.organism_classification ABA . IAA . Defence response . Fusarium oxysporum . rolA gene . Tomato 030104 developmental biology Fusarium oxysporum f.sp. lycopersici chemistry Solanum Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Journal of plant biochemistry and biotechnology (Online) (2015). doi:10.1007/s13562-015-0328-4 info:cnr-pdr/source/autori:Bettini PP., Santangelo E., Baraldi R., Rapparini F., Mosconi P., Crinò P., Mauro ML. 2015/titolo:Agrobacterium rhizogenes rolA gene promotes tolerance to Fusarium oxysporum f. sp. lycopersici in transgenic tomato plants (Solanum lycopersicum L.)./doi:10.1007%2Fs13562-015-0328-4/rivista:Journal of plant biochemistry and biotechnology (Online)/anno:2015/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| ISSN: | 0974-1275 0971-7811 |
| DOI: | 10.1007/s13562-015-0328-4 |
| Popis: | In order to assess the role of Agrobacterium rhizogenes rol genes on the defence response of plants to pathogens, tomato plants (Solanum lycopersicum L.) were transformed with the rolA gene. Consistently with previous descriptions of rolA-induced phenotype, insertion of this gene had a pleiotropic effect determining highly aberrant plants, with wrinkled, intensely green leaves, thick stems and small fruits often lacking seeds. Infection of transgenic plants with the phytopathogenic fungus Fusarium oxysporum f. sp. lycopersici showed the acquirement of resistance/tolerance to the pathogen as evaluated both on the primary transformants by electrolyte leakage and on the transgenic progenies by direct infection. Determination of the endogenous levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) showed a 30–35 % decrease of both phytohormones in rolA plants harbouring three copies of the transgene compared to the controls, while a significantly lower level of ABA was observed in plants with one copy of the transgene. This is the first demonstration of the direct involvement of rolA gene in plant pathogen tolerance acquisition. © 2015, Society for Plant Biochemistry and Biotechnology. |
| Databáze: | OpenAIRE |
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