A Biomimetic Lipid Membrane Device Reveals the Interaction of Cancer Biomarkers with Human Serum Lipidic Moieties

Autor: Abir Khalil-Mgharbel, Paul K. Dembélé, Isabelle Vilgrain, Jean-Pierre Alcaraz, Md. Mehadi Hasan Sohag, Donald K. Martin, Helena Polena
Přispěvatelé: Laboratoire de développement et vieillissement de l'endothélium, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Systèmes Nanobiotechnologiques et Biomimétiques (TIMC-IMAG-SyNaBi), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Alcaraz, Jean-Pierre
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Lipoproteins
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
[SDV]Life Sciences [q-bio]
Population
[SDV.CAN]Life Sciences [q-bio]/Cancer
Applied Microbiology and Biotechnology
03 medical and health sciences
0302 clinical medicine
[SDV.CAN] Life Sciences [q-bio]/Cancer
Antigens
CD
Biomimetic Materials
Biomarkers
Tumor
medicine
Humans
Immunoprecipitation
Nanotechnology
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
education
Lipid bilayer
ComputingMilieux_MISCELLANEOUS
education.field_of_study
Apolipoprotein A-I
biology
Chemistry
Cancer
General Medicine
Models
Theoretical
Cadherins
medicine.disease
Kidney Neoplasms
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
3. Good health
[SDV] Life Sciences [q-bio]
Cholesterol
HEK293 Cells
030104 developmental biology
Biochemistry
030220 oncology & carcinogenesis
biology.protein
Molecular Medicine
Biomarker (medicine)
lipids (amino acids
peptides
and proteins)
Apolipoprotein A1
Cancer biomarkers
Ultracentrifuge
Biotechnology
Lipoprotein
Zdroj: Biotechnology Journal
Biotechnology Journal, 2018, 13 (12), pp.1800463
Biotechnology Journal, Wiley-VCH Verlag, 2018, 13 (12), pp.1800463
HAL
ISSN: 1860-6768
1860-7314
Popis: A major problem for the detection of cancer biomarkers in plasma or serum is that common clinical practice does not require the patient to be in a fasting state. Considering that lipoproteins are the main population affected by food intake, the authors hypothesized that biomarkers could be embedded in lipid particles and thereby opens a new avenue for detection. Using the recently published biomarker, soluble VE-cadherin (sVE), the authors tested our hypothesis using techniques of biophysics, biochemistry and the tools of nanobiotechnology on serum samples from kidney cancer patients (n = 106). Optical density as well as contact angle measurements of serum revealed heterogeneity in the particle content of the serum samples. Isolation of the lipidic moieties by ultracentrifugation showed that sVE was detected in this compartment. Further, isolation of lipoprotein subclasses by precipitation with sodium phosphotungstate and MgCl2 , showed that HDL carried the majority of sVE. Immunoprecipitation of sVE confirmed that it was associated with Apolipoprotein A1, a major compound of HDL. Using a biomimetic lipid bilayer membrane coupled with impedance spectroscopy the authors quantified, in real-time, that the sVE adsorbed to the lipid bilayer membrane without altering its structure. Taken together, these results show for the first time a direct interaction of a cancer biomarker with lipids. The authors anticipate these results to prompt fasting for future blood tests for large-scale studies in the biomarkers research field.
Databáze: OpenAIRE
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