Recent advances in re-engineered liver: de-cellularization and re-cellularization techniques
| Autor: | Li-Ren Wang, Jiang-Tao Wang, Li Wan, Ka-Te Huang, Ming-Hua Zheng, Yi-Qian Lin, Gui-Qi Zhu, Liang-Liang Pan, Wen-Yue Liu, Martin Braddock |
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| Rok vydání: | 2015 |
| Předmět: |
Organ engineering
Cancer Research Pathology medicine.medical_specialty Allogeneic transplantation Immunology Rat model Biology Bioinformatics End Stage Liver Disease Liver disease medicine Animals Humans Transplantation Homologous Immunology and Allergy Donor shortage Genetics (clinical) Transplantation Tissue Engineering Regeneration (biology) Cell Biology medicine.disease Tissue Donors Extracellular Matrix Liver Regeneration Liver Transplantation Rats Liver Oncology Hepatocytes Cellularization |
| Zdroj: | Cytotherapy. 17:1015-1024 |
| ISSN: | 1465-3249 |
| DOI: | 10.1016/j.jcyt.2015.04.003 |
| Popis: | Allogeneic transplantation is the definitive treatment for patients with end-stage liver disease but is limited by donor shortage and very high cost. Through de-cellularization and re-cellularization methods, re-engineered liver may provide a promising alternative for treating patients with end-stage liver disease. To achieve this, the prevention of the native extracellular matrix ultrastructure plays a central role in de-cellularization protocol; the re-seeding cell types, as well as re-seeding strategies, need more explorations in re-cellularization protocol. Some success of this approach has been published in a rat model; however, the re-engineered liver remains functional in vivo for only several hours, which suggests that the recent protocol may be far from the ideal target. This Review highlights the challenges still to be overcome and presents an overview and summary of methods of de-cellularization and re-cellularization strategies, together with a view on future directions that may lead to the regeneration of a functional liver. |
| Databáze: | OpenAIRE |
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