The role of water channel proteins and nitric oxide signaling in rice seed germination
| Autor: | Da-Yong Cui, Weining Sun, Zhangcheng Tang, Xin Yu, Wei-Ai Su, Sang-Soo Kwak, Mei-Hao Sun, Hongyan Liu |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Nitroprusside
Blotting Western Aquaporin Germination Aquaporins Nitric Oxide Benzoates Nitric oxide chemistry.chemical_compound Gene Expression Regulation Plant Botany medicine Gene silencing Nitric Oxide Donors Molecular Biology Cyanides Oryza sativa biology Reverse Transcriptase Polymerase Chain Reaction Imidazoles Water food and beverages Oryza Embryo Cell Biology biology.organism_classification Cell biology chemistry Seedling S-Nitrosoglutathione Seeds Sodium nitroprusside Abscisic Acid Signal Transduction medicine.drug |
| Zdroj: | Cell Research. 17:638-649 |
| ISSN: | 1748-7838 1001-0602 |
| DOI: | 10.1038/cr.2007.34 |
| Popis: | Previous studies have demonstrated the possible role of several aquaporins in seed germination. But systematic investigation of the role of aquaporin family members in this process is lacking. Here, the developmental regulation of plasma membrane intrinsic protein (PIP) expression throughout germination and post-germination processes in rice embryos was analyzed. The expression patterns of the PIPs suggest these aquaporins play different roles in seed germination and seedling growth. Partial silencing of the water channel genes, OsPIP1;1 and OsPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions. Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination. Our data also revealed that the nitric oxide (NO) donors, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), promoted seed germination; furthermore, the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, inhibited germination and reduced the stimulative effects of SNP and GSNO on rice germination. Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds. These results suggest that water channels play an important role in seed germination, acting, at least partly, in response to the NO signaling pathway. |
| Databáze: | OpenAIRE |
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