Correction: Corrigendum: Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation
| Autor: | Edward A. Phelps, Chiara Cianciaruso, Jaime Santo-Domingo, Miriella Pasquier, Gabriele Galliverti, Lorenzo Piemonti, Ekaterine Berishvili, Olivier Burri, Andreas Wiederkehr, Jeffrey A. Hubbell, Steinunn Baekkeskov |
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| Přispěvatelé: | Pathology/molecular and cellular medicine |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male endocrine system Published Erratum Adolescent Organogenesis Cell Culture Techniques Hippocampus Microtubules Article Rats Sprague-Dawley Young Adult Insulin-Secreting Cells Journal Article Cell Adhesion Animals Humans Insulin Cilia Cells Cultured Cell Proliferation Neurons Microscopy Multidisciplinary Cell Differentiation Middle Aged Corrigenda Actins Coculture Techniques Glucose Phenotype Calcium Female Glass |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep46801 |
| Popis: | A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and live-cell microscopy. Guided by an observation that dispersed islet cells spread and adhere well on glass surfaces in neuronal co-culture and form a monolayer of connected cells, we demonstrate that in the absence of neurons, well-defined surface coatings combined with components of neuronal culture media collectively support robust attachment and growth of primary human or rat islet cells as monolayers on glass surfaces. The islet cell monolayer cultures on glass stably maintain distinct mono-hormonal insulin+, glucagon+, somatostatin+ and PP+ cells and glucose-responsive synchronized calcium signaling as well as expression of the transcription factors Pdx-1 and NKX-6.1 in beta cells. This technical advance enabled detailed observation of sub-cellular processes in primary human and rat beta cells by super-resolution microscopy. The protocol is envisaged to have broad applicability to sophisticated analyses of pancreatic islet cells that reveal new biological insights, as demonstrated by the identification of an in vitro protocol that markedly increases proliferation of primary beta cells and is associated with a reduction in ciliated, ostensibly proliferation-suppressed beta cells. |
| Databáze: | OpenAIRE |
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