v-SNARE-dependent secretion is required for phagocytosis
| Autor: | William S. Trimble, Alan D. Schreiber, Ori D. Rotstein, Sergio Grinstein, David J. Hackam, Carola Sjolin |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Botulinum Toxins
Phagocyte Phagocytosis media_common.quotation_subject Vesicular Transport Proteins CHO Cells Biology Exocytosis Cell Line Cell membrane R-SNARE Proteins Mice Tetanus Toxin Cricetinae medicine Leukocytes Animals Secretion Internalization Phagosome media_common Multidisciplinary Cell Membrane Membrane Proteins Transfection Biological Sciences Cell biology medicine.anatomical_structure SNARE Proteins |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 95(20) |
| ISSN: | 0027-8424 |
| Popis: | Phagosomes are generally believed to form by gradual apposition of the plasma membrane of leukocytes onto the surface of invading microorganisms. The internalization of the encapsulated particle is therefore predicted to reduce the surface area of the phagocyte. Contrary to this prediction, we observed that phagocytosis is associated with a net increase in cell surface area, suggesting the concomitant occurrence of exocytosis. Selective cleavage of components of the secretory machinery by microinjection or transfection of bacterial neurotoxins induced a pronounced inhibition of phagocytosis. These observations indicate that vesicle-solubleN-ethylmaleimide-sensitive factor attachment protein receptor-mediated exocytosis of endomembranes is essential for optimal completion of particle internalization during phagocytosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|