Visualization and Quantification of IgG Antibody Adsorbed at the Cellulose–Liquid Interface
| Autor: | Peter J. Holden, Jielong Su, Vikram Singh Raghuwanshi, Stephen A. Holt, Christopher J. Garvey, Warren Batchelor, Gil Garnier |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Polymers and Plastics Silicon Analytical chemistry chemistry.chemical_element Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Adsorption Materials Chemistry Animals Cellulose Thin film chemistry.chemical_classification Spin coating Biomolecule Substrate (chemistry) Membranes Artificial 021001 nanoscience & nanotechnology Cellulose acetate 0104 chemical sciences chemistry Chemical engineering Immunoglobulin G 0210 nano-technology |
| Zdroj: | Biomacromolecules. 18:2439-2445 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.7b00593 |
| Popis: | Quantification of adsorbed biomolecules (enzymes, proteins) at the cellulose interface is a major challenge in developing eco-friendly biodiagnostics. Here, a novel methodology is developed to visualize and quantify the adsorption of antibody from solution to the cellulose–liquid interface. The concept is to deuterate cellulose by replacing all nonexchangeable hydrogens from the glucose rings with deuterium in order to enhance the scattering contrast between the cellulose film surface and adsorbed antibody molecules. Deuterated cellulose (DC) was obtained from bacterial (Gluconacetobacter xylinus strain) cellulose, which was grown in heavy water (D2O) media with a deuterated glycerol as a carbon source. For comparison, hydrogenated cellulose (HC) was obtained from cellulose acetate. Both HC and DC thin films were prepared on silicon substrate by spin coating. X-ray reflectivity (XR) shows the formation of homogeneous and smooth film. Neutron reflectivity (NR) at the liquid/film interface reveals swelling ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|