Immunohistochemical Characteristics of Surfactant Proteins A, B, C and D in Inflammatory and Tumorigenic Lung Lesions of F344 Rats
| Autor: | Keiko Yamakawa, Sosuke Kishi, Hiroko Hitotsumachi, Shohei Kanie, Katsumi Imaida, Fumio Furukawa, Fumiko Ninomiya, Kousuke Saoo, Masanao Yokohira, Yuko Nakano, Takamasa Numano |
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| Rok vydání: | 2014 |
| Předmět: |
tumor
Pathology medicine.medical_specialty Inflammation Toxicology medicine.disease_cause lung Pathology and Forensic Medicine chemistry.chemical_compound Pulmonary surfactant Medicine rat Respiratory system urfactant protein Lung business.industry respiratory system quartz Mucus respiratory tract diseases medicine.anatomical_structure chemistry Nitrosamine Immunohistochemistry Original Article DHPN medicine.symptom business Carcinogenesis |
| Zdroj: | Journal of Toxicologic Pathology |
| ISSN: | 1881-915X 0914-9198 |
| Popis: | Surfactant proteins (SPs), originally known as human lung surfactants, are essential to respiratory structure and function. There are 4 subtypes, SP-A, SP-B, SP-C and SP-D, with SP-A and SP-D having immunological functions, and SP-B and SP-C having physicochemical properties that reduce the surface tension at biological interfaces. In this experiment, the expressions of SP-A, SP-B, SP-C and SP-D in lung neoplastic lesions induced by N-bis (2-hydroxypropyl) nitrosamine (DHPN) and inflammatory lesions due to quartz instillation were examined and compared immunohistochemically. Formalin fixed paraffin embedded (FFPE) lung samples featuring inflammation were obtained with a rat quartz instillation model, and neoplastic lesions, hyperplasias and adenomas, were obtained with the rat DHPN-induced lung carcinogenesis model. In the rat quartz instillation model, male 10-week old F344 rats were exposed by intratracheal instillation (IT) to quartz at a dose of 2 mg/rat suspended in saline (0.2 ml) on day 0, and sacrificed on day 28. Lung tumorigenesis in F344 male rats was initiated by DHPN in drinking water for 2 weeks, and the animals were then sacrificed in week 30. Lung proliferative lesions, hyperplasias and adenomas, were observed with DHPN, and inflammation was observed with quartz. The expressions of SP-A, SP-B, SP-C and SP-D were examined immunohistochemically. SP-B and SP-C showed strong expression in lung hyperplasias and adenomas, while SP-A and SP-D were observed in mucus or exudates in inflammatory alveoli. These results suggest the possibility that SP-B and SP-C are related to lung tumorigenesis. |
| Databáze: | OpenAIRE |
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