The expression of sonic hedgehog in diabetic wounds following treatment with poly(methacrylic acid-co-methyl methacrylate) beads

Autor: Alexandra Lisovsky, Lindsay E. Fitzpatrick, Michael V. Sefton
Rok vydání: 2012
Předmět:
Blood Glucose
Male
Poly(methacrylic acid)
Materials science
Biophysics
Kruppel-Like Transcription Factors
Neovascularization
Physiologic
Bioengineering
Nerve Tissue Proteins
030204 cardiovascular system & hematology
Methacrylate
Real-Time Polymerase Chain Reaction
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Zinc Finger Protein Gli3
otorhinolaryngologic diseases
medicine
Diabetes Mellitus
Animals
Polymethyl Methacrylate
Hedgehog Proteins
Osteopontin
Sonic hedgehog
Methyl methacrylate
030304 developmental biology
0303 health sciences
Wound Healing
integumentary system
biology
Body Weight
Granulation tissue
Molecular biology
Microspheres
3. Good health
medicine.anatomical_structure
chemistry
Methacrylic acid
Gene Expression Regulation
Mechanics of Materials
Ceramics and Composites
biology.protein
Granulation Tissue
Blood Vessels
Cytokines
Wound healing
Biomedical engineering
Zdroj: Biomaterials. 33(21)
ISSN: 1878-5905
Popis: The expression of native sonic hedgehog (Shh) was significantly increased in poly(methacrylic acid-co-methyl methacrylate) bead (MAA) treated wounds at day 4 compared to both poly(methyl methacrylate) bead (PMMA) treated and untreated wounds in diabetic db/db mice. MAA beads also increased the expression of the Shh transcription factor Gli3 at day 4. Previously, topical application of MAA beads (45 mol % methacrylic acid) improved wound closure and blood vessel density in excisional wounds in these mice, while PMMA beads did not. Gene expression within the granulation tissue of healing wounds was studied to provide insight into the mechanism of vessel formation and wound healing in the presence of MAA beads. In addition to the increased expression of Shh, MAA-treated wounds had increased expression of osteopontin (OPN), IL-1β and TNF-α, (at day 7) similar to the previously reported MAA response of macrophage-like and endothelial cells in vitro.
Databáze: OpenAIRE
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