Overexpression of the Squalene Epoxidase Gene Alone and in Combination with the 3-Hydroxy-3-methylglutaryl Coenzyme A Gene Increases Ganoderic Acid Production in Ganoderma lingzhi
| Autor: | Na Li, Jun-Wei Xu, Xuya Yu, Tao Li, Peng Zhao, De-Huai Zhang, Lu-Xi Jiang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Squalene monooxygenase Ganoderma Coenzyme A Gene Expression Fungal Proteins 03 medical and health sciences Squalene chemistry.chemical_compound Biosynthesis Gene expression biology Ganoderic acid General Chemistry biology.organism_classification Triterpenes Biosynthetic Pathways 030104 developmental biology chemistry Biochemistry Squalene Monooxygenase biology.protein Acyl Coenzyme A General Agricultural and Biological Sciences Lanosterol synthase |
| Zdroj: | Journal of agricultural and food chemistry. 65(23) |
| ISSN: | 1520-5118 |
| Popis: | The squalene epoxidase (SE) gene from the biosynthetic pathway of ganoderic acid (GA) was cloned and overexpressed in Ganoderma lingzhi. The strain that overexpressed the SE produced approximately 2 times more GA molecules than the wild-type (WT) strain. Moreover, SE overexpression upregulated lanosterol synthase gene expression in the biosynthetic pathway. These results indicated that SE stimulates GA accumulation. Then, the SE and 3-hydroxy-3-methylglutaryl coenzyme A (HMGR) genes were simultaneously overexpressed in G. lingzhi. Compared with the individual overexpression of SE or HMGR, the combined overexpression of the two genes further enhanced individual GA production. The overexpressing strain produced maximum GA-T, GA-S, GA-Mk, and GA-Me contents of 90.4 ± 7.5, 35.9 ± 5.4, 6.2 ± 0.5, and 61.8 ± 5.8 μg/100 mg dry weight, respectively. These values were 5.9, 4.5, 2.4, and 5.8 times higher than those produced by the WT strain. This is the first example of the successful manipulation of multiple biosynthetic genes to improve GA content in G. lingzhi. |
| Databáze: | OpenAIRE |
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