Genistein Increases the Sensitivity of Cardiac Ion Channels to β-Adrenergic Receptor Stimulation
| Autor: | Robert D. Harvey, Livia C. Hool, Lisa M. Middleton |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Agonist
medicine.medical_specialty Patch-Clamp Techniques Physiology medicine.drug_class Guinea Pigs Cystic Fibrosis Transmembrane Conductance Regulator Genistein Stimulation Protein tyrosine phosphatase Ion Channels Tyrosine-kinase inhibitor chemistry.chemical_compound Chlorides Internal medicine Cyclic AMP medicine Animals Enzyme Inhibitors biology Myocardium Daidzein Electric Conductivity Isoproterenol Adrenergic beta-Agonists Protein-Tyrosine Kinases Endocrinology chemistry Enzyme inhibitor Potassium biology.protein Calcium Cardiology and Cardiovascular Medicine Tyrosine kinase |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/01.res.83.1.33 |
| Popis: | Abstract —The whole-cell patch-clamp technique was used to monitor the effects of genistein, a tyrosine kinase inhibitor, on membrane currents recorded from isolated guinea pig ventricular myocytes. Under control conditions, genistein (50 μmol/L) did not activate the latent cAMP-regulated Cl − current ( I Cl ). However, in the presence of a subthreshold concentration (1 nmol/L) of the β-adrenergic agonist isoproterenol (Iso), genistein caused a near-maximal activation of this current. In the absence of genistein, Iso activated I Cl with an EC 50 of 5 nmol/L. In the presence of genistein, Iso activated I Cl with an EC 50 of 0.3 nmol/L. This facilitatory effect was not observed in the presence of daidzein (50 μmol/L), an analogue of genistein that only weakly inhibits tyrosine kinase activity. Furthermore, peroxovanadate, a potent inhibitor of phosphotyrosine phosphatase activity, inhibited I Cl activated by Iso alone, and it blocked the stimulatory effect of genistein in the presence of Iso. To determine whether the stimulatory effect of genistein was specific for I Cl , we also studied its action on the cAMP-regulated delayed rectifier K + current ( I K ) and L-type Ca 2+ current ( I Ca-L ) present in these cells. Basal I K and I Ca-L were partially (≈30% to 40%) inhibited by genistein. However, this inhibitory effect was mimicked by daidzein, suggesting that inhibition of tyrosine kinase activity is not involved. In addition to the nonspecific inhibitory effect, genistein also caused a significant increase in the β-adrenergic sensitivity of the unblocked cationic currents. In the absence of genistein, 1 nmol/L Iso had no effect on either I K or I Ca-L . However, in the presence of genistein, 1 nmol/L Iso significantly increased the magnitude of both currents. These results suggest that tyrosine kinase activity may play an important role in regulating β-adrenergic responsiveness of the heart. |
| Databáze: | OpenAIRE |
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