cDNA cloning, expression and immune function analysis of a novel Rac1 gene ( AjRac1 ) in the sea cucumber Apostichopus japonicus
| Autor: | Yi Wang, Kaiquan Li, Yaoyao Zhan, Lin Liu, Shengnan Shang, Jian Song, Xiangxiang Zhang, Yaqing Chang |
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| Rok vydání: | 2017 |
| Předmět: |
rac1 GTP-Binding Protein
0301 basic medicine DNA Complementary Sequence analysis Gene Expression Aquatic Science 03 medical and health sciences Sea cucumber Rapid amplification of cDNA ends Botany Animals Environmental Chemistry Amino Acid Sequence Cloning Molecular Gene Peptide sequence Phylogeny Genetics Base Sequence biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Strongylocentrotus purpuratus Immunity Innate Open reading frame 030104 developmental biology Gene Expression Regulation Stichopus Apostichopus japonicus 040102 fisheries 0401 agriculture forestry and fisheries Sequence Alignment Signal Transduction |
| Zdroj: | Fish & Shellfish Immunology. 69:218-226 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2017.08.027 |
| Popis: | The ras-related C3 botulinum toxin substrate 1 (Rac1) belongs to Ras homolog (Rho) small GTPases subfamily. As an important molecular switch, Rac1 regulates various processes in the cell, especially in cellular immune response. With attempt to clarify characters and functions of Rac1 in sea cucumbers, full length cDNA of a Rac1 homolog in the sea cucumber Apostichopus japonicus (AjRac1) was cloned by transcriptome database mining and rapid amplification of cDNA ends (RACE) techniques. The open reading frame of AjRac1 is 579 bp encoding a protein with a length of 192 aa. Sequence analysis showed that AjRac1 is highly conserved as compared to those from other eukaryotic species. Phylogenetic analysis revealed that amino acid sequence of AjRac1 closely related to those from Strongylocentrotus purpuratus. Results of expression analysis showed that AjRac1 exhibited a relative high expression in blastula stage, adult coelomocytes and respiratory tree in A. japonicus. The transcription of AjRac1 in adult coelomocytes altered significantly at 4 h- and 12 h-after Vibrio splendidus infection, respectively, which indicated that AjRac1 involved in sea cucumber innate immunity. All data presented in this study will deepen our understanding of characterizations and immunological functions of Rac1 in sea cucumbers. |
| Databáze: | OpenAIRE |
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