Tubular Deficiency of von Hippel-Lindau Attenuates Renal Disease Progression in Anti-GBM Glomerulonephritis
| Autor: | Franziska Theilig, Sebastian Bachmann, Robert Koesters, Danny Polzin, Anne Kathrin Enke, Brigitte Scolari |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty von Hippel-Lindau Disease Anti-Glomerular Basement Membrane Disease Kidney Glomerulus Neovascularization Physiologic Biology urologic and male genital diseases Pathology and Forensic Medicine Kidney Tubules Proximal Mice Interstitial matrix Fibrosis Internal medicine medicine Animals RNA Messenger Autoantibodies Cell Proliferation Mice Knockout Kidney urogenital system Regular Article Glomerulonephritis Proto-Oncogene Proteins c-sis Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Immunohistochemistry female genital diseases and pregnancy complications Capillaries Vascular endothelial growth factor A Endocrinology medicine.anatomical_structure Von Hippel-Lindau Tumor Suppressor Protein Disease Progression Cancer research medicine.symptom Tubulointerstitial Disease |
| Zdroj: | The American Journal of Pathology. 179:2177-2188 |
| ISSN: | 0002-9440 |
| Popis: | In many kidney diseases, the original insult primarily involves the glomerulus and may then pass onto the tubulointerstitium. Several hypotheses link glomerular disease to tubular injury; perhaps the foremost hypothesis involves chronic tubular hypoxia. The reported effects of hypoxia and consecutive stabilization of hypoxia-inducible factors (HIFs), however, are controversial. Hypoxia induces interstitial fibrosis but also has beneficial effects on renal disease progression when HIF is activated pharmacologically. To analyze the impact of HIF on tubulointerstitial disease development in primary glomerular disease, transgenic von Hippel Lindau (VHL)-knockout mice were generated and null expression was induced before the onset of autoimmune IgG-mediated anti-glomerular basement membrane glomerulonephritis (GN). Tubular VHL knockout and, thus, local HIF-α stabilization increased renal production of vascular endothelial growth factor, tumor growth factor-β(1), and platelet-derived growth factor-B, resulting in augmented formation of capillaries and interstitial matrix, and conversion of fibroblasts to myofibroblasts. Within the glomerular disease, VHL knockout reduced the glomerular damage and attenuated tubulointerstitial injury. Likewise, proteinuria, plasma urea concentration, and tubulointerstitial matrix were decreased in VHL knockout with GN. These findings shown that tubular HIF-α stabilization in glomerular disease is beneficial for disease outcome. In comparison with VHL knockout alone, GN is a much stronger activator of fibrosis such that stimuli other than hypoxia may be considered important for renal disease progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|