An assay for glucose 6-phosphatase based on the formation of glucose
| Autor: | Clark Bublitz |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Clinical Biochemistry
Tritium Potassium Chloride chemistry.chemical_compound Methods Animals Carbon Radioisotopes Molecular Biology Anion Exchange Resins chemistry.chemical_classification Chromatography biology Substrate (chemistry) Cell Biology General Medicine Rats Kinetics Resins Synthetic Glucose Enzyme Glucose 6-phosphate chemistry Glucose-6-Phosphatase Slurry Microsome biology.protein Quantitative analysis (chemistry) Fluoride Glucose 6-phosphatase |
| Zdroj: | Molecular and Cellular Biochemistry. 108 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/bf00233118 |
| Popis: | A rapid, convenient method for the assay of glucose 6-phosphatase dependent on the removal of radioactive substrate from radioactive product by Dowex 2 fluoride is described. The enzymatic reaction is stopped by the addition of an ethanolic slurry of the resin. After the tubes are shaken, the radioactivity of glucose in the clarified supernatant layer is measured. The release of glucose is directly proportional to time and enzyme concentration. Detergents do not interfere with the method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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