Evaluation of methods for the isolation, detection and quantification of cyanobacterial hepatotoxins
| Autor: | Deborah D Shushu, Bupe A. Siame, Titus A.M. Msagati |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cyanobacteria
Microcystins Health Toxicology and Mutagenesis Chemical structure Bacterial Toxins Enzyme-Linked Immunosorbent Assay Aquatic Science Peptides Cyclic High-performance liquid chromatography Gas Chromatography-Mass Spectrometry Animals Humans Nuclear Magnetic Resonance Biomolecular Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography biology Extraction (chemistry) Hepatotoxin Electrophoresis Capillary Environmental Exposure Eutrophication biology.organism_classification Enzyme assay Enzyme Liver chemistry biology.protein Marine Toxins Gas chromatography–mass spectrometry Water Microbiology |
| Zdroj: | Aquatic Toxicology. 78:382-397 |
| ISSN: | 0166-445X |
| DOI: | 10.1016/j.aquatox.2006.03.011 |
| Popis: | Cyanobacterial hepatotoxins such as microcystins and nodularins have been responsible for the poisoning of both animals and humans who ingest or come into contact with toxic blooms. They are extremely stable in water due to their stable chemical structure and can tolerate radical changes in water chemistry, including pH and salinity. Different methods for the extraction and detection of these compounds have been reported. Extraction methods utilizing both aqueous and organic solvent systems have been reported. The detection methods ranging from immunological or biochemical assays such as enzyme linked immunosorbent assays (ELISA) and enzyme activity assays, to chemicals methods such as high performance liquid chromatography (HPLC) and more sophisticated liquid chromatography-mass spectrometry (LC-MS) have been documented as well. We review some important aspects of cyanobacterial hepatotoxins and methods of analysis for these toxins. |
| Databáze: | OpenAIRE |
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