Real-time magnetic actuation of DNA nanodevices via modular integration with stiff micro-levers
| Autor: | Maxim Armstrong, Thomas P. Rudibaugh, Ratnasingham Sooryakumar, Carlos E. Castro, Alexander E. Marras, Stephanie Lauback, Kara R. Mattioli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
business.product_category Computer science Surface Properties Science Video Recording General Physics and Astronomy DNA Single-Stranded Nanotechnology 02 engineering and technology Microscopy Atomic Force General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Magnetics Microscopy Electron Transmission Oscillometry DNA nanotechnology DNA origami Nanobiotechnology lcsh:Science Nanodevice Lever Multidisciplinary business.industry Control reconfiguration General Chemistry Modular design 021001 nanoscience & nanotechnology Molecular machine Nanostructures 030104 developmental biology Nucleic Acid Conformation lcsh:Q 0210 nano-technology business |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | DNA nanotechnology has enabled complex nanodevices, but the ability to directly manipulate systems with fast response times remains a key challenge. Current methods of actuation are relatively slow and only direct devices into one or two target configurations. Here we report an approach to control DNA origami assemblies via externally applied magnetic fields using a low-cost platform that enables actuation into many distinct configurations with sub-second response times. The nanodevices in these assemblies are manipulated via mechanically stiff micron-scale lever arms, which rigidly couple movement of a micron size magnetic bead to reconfiguration of the nanodevice while also enabling direct visualization of the conformation. We demonstrate control of three assemblies—a rod, rotor, and hinge—at frequencies up to several Hz and the ability to actuate into many conformations. This level of spatiotemporal control over DNA devices can serve as a foundation for real-time manipulation of molecular and atomic systems. DNA molecular machines hold promise for biological nanotechnology, but how to actuate them in a fast and programmable manner remains challenging. Here, Lauback et al. demonstrate direct manipulation of DNA origami assemblies via a micrometer-long stiff mechanical lever controlled by a magnetic field. |
| Databáze: | OpenAIRE |
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