A Cluster of Five Genes Essential for the Utilization of Dihydroxamate Xenosiderophores in Synechocystis sp. PCC 6803
| Autor: | M. M. Babykin, A S Tobias Obando, Vladislav V. Zinchenko |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Siderophore Iron Siderophores Hydroxamic Acids Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Bacterial Proteins INDEL Mutation Gene cluster Anabaena variabilis Siderophore transport biology Permease Genetic Complementation Test Synechocystis Membrane Transport Proteins Biological Transport General Medicine Periplasmic space biology.organism_classification Transmembrane protein 030104 developmental biology Biochemistry Multigene Family bacteria Bacterial outer membrane |
| Zdroj: | Current microbiology. 75(9) |
| ISSN: | 1432-0991 |
| Popis: | The unicellular freshwater cyanobacterium Synechocystis sp. PCC 6803 is capable of using dihydroxamate xenosiderophores, either ferric schizokinen (FeSK) or a siderophore of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 (SAV), as the sole source of iron in the TonB-dependent manner. The fecCDEB1-schT gene cluster encoding a siderophore transport system that is involved in the utilization of FeSK and SAV in Synechocystis sp. PCC 6803 was identified. The gene schT encodes TonB-dependent outer membrane transporter, whereas the remaining four genes encode the ABC-type transporter FecB1CDE formed by the periplasmic binding protein FecB1, the transmembrane permease proteins FecC and FecD, and the ATPase FecE. Inactivation of any of these genes resulted in the inability of cells to utilize FeSK and SAV. Our data strongly suggest that Synechocystis sp. PCC 6803 can readily internalize Fe-siderophores via the classic TonB-dependent transport system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink