Chitin synthase-encoding gene(s) of the Zygomycete fungus Phycomyces blakesleeanus
| Autor: | Miyazaki A, Momany M, Paul J. Szaniszlo, Ootaki T, Makkuni Jayaram |
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| Rok vydání: | 1993 |
| Předmět: |
Genes
Fungal Molecular Sequence Data Biology Conserved sequence Phycomyces hemic and lymphatic diseases Genetics Amino Acid Sequence Cloning Molecular DNA Fungal skin and connective tissue diseases Gene Conserved Sequence Southern blot Chitin Synthase Base Sequence Sequence Homology Amino Acid integumentary system Nucleic acid sequence General Medicine Chitin synthase biology.organism_classification genomic DNA biology.protein Phycomyces blakesleeanus |
| Zdroj: | Gene. 134:129-134 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(93)90186-7 |
| Popis: | Using polymerase chain reaction (PCR) primers to two highly conserved sequences within fungal chitin synthase (CHS)-encoding genes, an approximately 750-bp DNA fragment was amplified from genomic DNA of Phycomyces blakesleeanus. The amino acid sequence deduced from the nucleotide sequence of this fragment best matches the motifs found in class-II CHS. The fragment includes an approximately 160-bp region that likely is an intron. Southern hybridization of restriction enzyme-digested genomic DNA, using the PCR-amplified DNA as the probe, suggests that P. blakesleeanus contains additional CHS-encoding genes (CHS). To our knowledge, this is the first report on the detection of a CHS gene in a Zygomycete fungus. These studies represent a major step toward exploring the molecular mechanisms of CHS regulation in Phycomyces. The prospects are exciting, since CHS is implicated to play a central role in the sensory responses of P. blakesleeanus involving growth modulations. |
| Databáze: | OpenAIRE |
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