Thyroid hormone regulates the expression of α-internexin in neurons in culture
| Autor: | David Sampson, A.J. Leonard, AK Sinha, Mark R. Pickard, Ian M. Evans, Roger Ekins |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Thyroid Hormones medicine.medical_specialty Blotting Western Nerve Tissue Proteins Biology Rats Sprague-Dawley Maternal hypothyroidism Intermediate Filament Proteins Pregnancy Internal medicine Gene expression medicine Animals Microscopy Phase-Contrast RNA Messenger Receptor Cells Cultured Neurons Receptors Thyroid Hormone Triiodothyronine Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Thyroid Brain Cell Differentiation DNA medicine.disease Immunohistochemistry Rats medicine.anatomical_structure Endocrinology Nuclear receptor Female Neuron Carrier Proteins Hormone |
| Zdroj: | Neuroreport. 13:273-276 |
| ISSN: | 0959-4965 |
| Popis: | Maternal hypothyroidism in the rat compromises alpha-internexin (alpha-IN) expression in early fetal brain. We have therefore examined whether 3,5,3'-triiodothyronine (T3) regulates alpha-IN expression in fetal brain neurons in culture. Cells expressed transcripts encoding T3 nuclear receptor isoforms in a T3-independent manner. alpha-IN protein abundance was increased in cultures treated with 0.1 and 1 nM T3 for 20 h (177 and 185% control, respectively) and in cultures treated with 1 nM T3 for 40 h (131% control). alpha-IN transcript abundance was unaffected by T3 treatment. In conclusion, T3 at a physiological level, stimulates alpha-IN protein, but not mRNA, levels in early differentiating neurons in culture. This supports the hypothesis that maternal thyroid hormone directly regulates early neuronal differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|