Filopodia are conduits for melanosome transfer to keratinocytes
| Autor: | Glynis Scott, Stacey Printup, Brian C. Madden, Sonya Leopardi |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Keratinocytes
Melanosomes Effector Cell Wiskott-Aldrich Syndrome Protein Neuronal Biological Transport Nerve Tissue Proteins macromolecular substances Cell Biology CDC42 Melanocyte Biology Cell biology Blot medicine.anatomical_structure Organelle Image Processing Computer-Assisted medicine Humans Pseudopodia cdc42 GTP-Binding Protein Protein Kinases Filopodia Cells Cultured Melanosome |
| Zdroj: | Journal of Cell Science. 115:1441-1451 |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | Melanosomes are specialized melanin-synthesizing organelles critical for photoprotection in the skin. Melanosome transfer to keratinocytes, which involves whole organelle donation to another cell, is a unique biological process and is poorly understood. Time-lapse digital movies and electron microscopy show that filopodia from melanocyte dendrites serve as conduits for melanosome transfer to keratinocytes. Cdc42, a small GTP-binding protein, is known to mediate filopodia formation. Melanosome-enriched fractions isolated from human melanocytes expressed the Cdc42 effector proteins PAK1 and N-WASP by western blotting. Expression of constitutively active Cdc42(Cdc42V12) in melanocytes co-cultured with keratinocytes induced a highly dendritic phenotype with extensive contacts between melanocytes and keratinocytes through filopodia, many of which contained melanosomes. These results suggest a unique role for filopodia in organelle transport and, in combination with our previous work showing the presence of SNARE proteins and rab3a on melanosomes, suggest a novel model system for melanosome transfer to keratinocytes. |
| Databáze: | OpenAIRE |
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