Axonal Transport of the Ca2+-Dependent Protein Modulator of 3':5'-Cyclic-AMP Phosphodiesterase in the Rabbit Visual System
| Autor: | Blake W. Moore, Lee N. Minier, Kenneth B. Seamon, Paul F. Erickson, Robert S. Lasher |
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| Rok vydání: | 1980 |
| Předmět: |
Superior Colliculi
Optic tract Calmodulin Biology Axonal Transport Biochemistry Retina Cellular and Molecular Neuroscience Leucine Animals Polyacrylamide gel electrophoresis Calcium-Binding Proteins Brain Geniculate Bodies Optic Nerve Rabbit (nuclear engineering) 3' 5'-cyclic-AMP phosphodiesterase Kinetics 3' 5'-Cyclic-AMP Phosphodiesterases Biophysics Axoplasmic transport biology.protein Calcium Electrophoresis Polyacrylamide Gel Rabbits |
| Zdroj: | Journal of Neurochemistry. 35:242-248 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1980.tb12510.x |
| Popis: | Water-soluble proteins were extracted from individual retinas, optic nerves, combined optic tracts and lateral geniculate bodies, and superior colliculi of rabbits at 1, 3, and 18 days after injection of [3H]leucine into the right eye. The Ca2+-dependent protein modulator of 3′:5′-cyclic-AMP phos-phodiesterase (calmodulin) was isolated from these samples by a two-step polyacrylamide gel electrophoresis procedure. An analysis of the radioactivity incorporated into the total soluble proteins and the calmodulin revealed that most of the calmodulin was axonally transported at a slow rate (2–4 mm/day) and represented about 0.45% of the total transported soluble protein. |
| Databáze: | OpenAIRE |
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