Facilitated Peptide Transport via the Mucosal Epithelium: Impact on Tolerance Induction
| Autor: | A. Pernthaner, Jennifer Pfeil, Ute Hoffmann, Anne Rigby, Anja A. Kühl, Alf Hamann, Uta Lauer, Elisabeth E. Kenngott |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Immunology Biology medicine.disease_cause regulatory T cells Autoimmunity 03 medical and health sciences 0302 clinical medicine Antigen medicine Immunology and Allergy mucosa Original Research Gastrointestinal tract Lamina propria Effector oral tolerance targeted delivery peptide vaccination Vaccination Tolerance induction 030104 developmental biology medicine.anatomical_structure Peptide transport mucosal uptake 030215 immunology |
| Zdroj: | Frontiers in Immunology |
| ISSN: | 1664-3224 |
| Popis: | A hallmark of autoimmunity is the breakdown of tolerance and generation of effector responses against self-antigens. A hallmark of autoimmunity is the breakdown of tolerance and generation of effector responses against self-antigens. Re-establishment of tolerance in autoimmune disorders was always the most desired treatment option; however, despite many efforts, clinical trials have been largely unsuccessful. This also applies to the generation of oral tolerance, which seems to be a default response type of the mucosa-associated lymphoid tissues (MALT) to harmless antigens. In this study we report improved efficacy of oral tolerance induction by coupling antigen with the newly identified mucosal carrier peptide 13C. Antigen coupled to 13C is efficiently taken up in the gastrointestinal tract and could be visualized in cells of the lamina propria. Oral, rectal or nasal treatment effectively induced the proliferation of antigen-specific T cells with some increase in the frequency of regulatory T cells. In a model of delayed type hypersensitivity, especially intrarectal tolerization treatment resulted in reduced footpad swelling, demonstrating a moderate tolerogenic effect of mucosal treatment with 13C coupled antigen. Coupling of antigens to a transmucosal carrier therefore is a promising tool to improve the efficacy of vaccination via mucosal surfaces. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|