Comparison of the in vitro anticollagenase efficacy of homologous serum and plasma on degradation of corneas of cats, dogs, and horses
| Autor: | Wendy M. Townsend, Hsin Yi Weng, Emily D. Conway, Jean Stiles |
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| Rok vydání: | 2016 |
| Předmět: |
Serum
Pathology medicine.medical_specialty 040301 veterinary sciences medicine.medical_treatment Matrix Metalloproteinase Inhibitors Biology Cornea 0403 veterinary science Andrology Plasma 03 medical and health sciences Hydroxyproline chemistry.chemical_compound Dogs 0302 clinical medicine Weight loss medicine Animals Collagenases Horses Incubation Saline CATS General Veterinary 04 agricultural and veterinary sciences General Medicine eye diseases In vitro medicine.anatomical_structure chemistry Cats 030221 ophthalmology & optometry Collagenase sense organs medicine.symptom medicine.drug |
| Zdroj: | American Journal of Veterinary Research. 77:627-633 |
| ISSN: | 0002-9645 |
| Popis: | OBJECTIVE To compare the anticollagenase efficacy of fresh feline, canine, and equine serum and plasma on in vitro corneal degradation. SAMPLE Grossly normal corneas from recently euthanized dogs, cats, and horses and fresh serum and plasma from healthy dogs, cats, and horses. PROCEDURES Serum and plasma were pooled by species and used for in vitro experiments. Corneas were collected and stored at −80°C. Sections of cornea were dried, weighed, and incubated in saline (0.9% NaCl) solution with clostridial collagenase and homologous fresh serum or plasma. Corneal degradation was assessed as the percentage of corneal weight loss and hydroxyproline concentration, compared with results for positive and negative control samples. RESULTS Homologous fresh serum and plasma significantly reduced the percentage of corneal weight loss, compared with results for positive control samples. No significant difference was found in percentage of corneal weight loss between incubation with serum or plasma for feline, canine, and equine corneas. Canine serum and plasma significantly reduced hydroxyproline concentrations, whereas inclusion of feline and equine serum or plasma did not, compared with results for positive control samples. Hydroxyproline concentrations were moderately correlated with percentage of corneal weight loss for feline samples and weakly correlated for equine samples, but they were not correlated for canine samples. CONCLUSIONS AND CLINICAL RELEVANCE In this study, the anticollagenase efficacy of fresh feline, canine, and equine serum was not different from that of plasma. Plasma should be an acceptable substitute for serum in the topical treatment of keratomalacia. |
| Databáze: | OpenAIRE |
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